If you're guessing this would leave the polymerase racing around and around the closed padlock probe template and creating an ever-lengthening nascent strand consisting of concatemer
linear copies of the circular sequence, you're right.
Similarly, a polymeric epitope concatemer
in the form of a "string-of-beads" could be chemically synthesized or formulated as a genetic construct .
After the single gene alignments, the sequences were joined to make a concatemer
of loci at head-to-tail inframe.
Rolling circle replication in solution was developed to form billions of discrete DNA nanoballs (DNBs), each comprising a concatemer
with approximately 500 copies of the original circle.
DNA was used as a template for the first PCR amplification, using forward primer 5'-CCTTTGTCTCTGGAGCCG-37 and reverse primer 5'-GGCTAACGTAGGGTCCAGCT-37.
However, deletions extending into regions adjacent to the transgene concatemer
are not detected, as two intact cos sites are required for the packaging of a single [lambda] vector.
Other groups have expressed recombinant T[alpha]1 as a fusion protein or in concatemer
form in E.
sequences were analyzed by using SAGE software (version 4.0; Invitrogen), which automatically detects and counts tags from sequence files.
Bacteriophage lambda concatemers
(Bio-Rad) were used as DNA size markers.
Lambda phage concatemers
(Bio-Rad Laboratories; USA) were used as a size marker.
VWF multimerization is a multistep process: monomers of VWF firstly dimerize in the endoplasmic reticulum (ER); then, they link in mature VWF multimers or "concatemers
" in the Golgi and post-Golgi compartments [7, 8].
With the magnetic induction intensity of external magnetic field increasing, the linear extent of flocculation grows obviously by developing from dimers or triplets into concatemers
with the occurrence of branched chains.