Comet Assay


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A rapid method for detecting DNA damage in individual cells, which is simple and faster than conventional genotoxicity techniques—e.g., cytogenetics. The name derives from use of the tail moment of the electropherogram for evaluating DNA damage, which is defined as the product of the tail length and the fraction of the total DNA content in the tail of the electropherogram
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To have a relatively thorough toxicity analysis of DMSA/[Ag.sub.2]S NIR QDs, the MTT and neutral red uptake (NRU) assays were performed to evaluate the potential cytotoxicity; the comet assay was performed to assess the potential genotoxicity; the real-time polymerase chain reaction (RT-PCR) technique was used to evaluate the regulation of mRNA expression of tumor suppressor gene (p53), apoptotic genes (caspase-3, caspase-9, and Bax) and anti-apoptotic genes (Bcl-2 and survivin).
At the molecular level, the comet assay and the micronucleus test (MN) are complementary biomarkers for genotoxic testing and indicate significant DNA damage based on two different endpoints (Rudolf and Cervinka, 2006; Villela et al., 2006; Medeiros et al., 2017).
de Assis et al carried out a study to assess DNA damage using the comet assay in lymphocytes of smoking and non-smoking mothers and their newborns.
Keywords: Cigarette smoking, Genotoxicity, Comet assay, Total comet score, Green tea.
After 24 hours of culture, an aliquot was taken for the accomplishment of the Comet Assay (T0), and the treatments were added, returning the flasks to the culture chamber for another 24 hours.
Tenders invited for Comet assay tank with accessories
DNA damage induced by indirect and direct acting mutagens in catalase-deficient transgenic tobacco: cellular and acellular comet assay. Mutat Res.
The genotoxicity potential of tilmicosin was accessed through comet assay. It is a valuable tool for the determination of DNA damage on individual cell.
The alkaline version of the comet assay was the method selected to measure both basal DNA damage and oxidative stress, since this method quantifies both endpoints.