They use the underlying principles to explain everything from small molecules to the collision-induced dissociation
of biological poly-peptides.
Data interpretation was challenging due to missing fragmentation information as well as low resolution and low mass accuracy data, so while the fragmentation of peptides in collision-induced dissociation
was understood, the rapid interpretation of the spectra was still problematic.
However, the fragmentation patterns of several classes of natural products under collision-induced dissociation
(CID) conditions have not been well-established yet, which has made building spectral libraries difficult and limited the use of LC-ESI-MS/MS for qualitative and quantitative studies on these compounds .
Abbreviations: PLD, Chinese peony-Liquorice Decoction; GA, glycyrrhetinic acid; PF, paeoniflorin; TTS, transdermal therapeutic system; PK, pharmacokinetics; PD, pharmacodynamics; PSB, phosphate buffer solution; HPLC, high-performance liquid chromatography; DM, dysmenorrhea model mice; NW, number of writhes exhibited by mice; PIR, pain inhibition ratio; AL, antalgic latency; BI, Bliss independence; UV, ultraviolet; LC-MS/MS, liquid chromatography tandem mass spectrometry; ESI, electrospray ionization; CID, collision-induced dissociation
; SRM, selected reaction monitoring; QC, quality control; [C.sub.max].
voltage was 22 eV for both the analyte and internal standard.
This is due to the fact that one can always utilize the collision-induced dissociation
(CID) in the QqQ which usually produces more abundant fragment ions than those shown in case of resonance excitation in the ion trap (IT) .
MS spectra were recorded from a range of m/z 350-1500 at 100,000 resolution, followed by data-dependent collision-induced dissociation
(CID) MS/MS spectra and electron transfer dissociation (ETD) MS/MS spectra generated from the 20 highest intensity precursor ions.
Recently, one of his major discoveries on the “Formation of the bisulfite anion (HSO3- , m/z 81) upon collision-induced dissociation
of anions derived from organic sulfonic acids” was accepted for publication in the Journal of Mass Spectrometry.
The result is high-efficiency collision-induced dissociation
(C1D) and ultra-fast ion transport, reducing the sensitivity losses and cross talk observed on other systems.
Their topics include human biotransformation, analytical tools and approaches for metabolic identification in drug metabolism, the unanticipated loss of N2 from novel DNA alkylating agent laromustine by collision-induced dissociation
, and strategies for detecting reactive intermediates in drug discovery and development.
According to the company, the result is high-efficiency collision-induced dissociation
(CID) and ultra-fast ion transport, reducing the sensitivity losses and cross talk observed on other systems.
This hybrid LC-MS approach also features various dissociation methods, including Higher-energy Collisional Dissociation in the HCD collision cell (Figure 1) and Collision-Induced Dissociation
in the linear ion trap.