Colcemid


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Colcemid

 [kol´sĕ-mid]
trademark for a preparation of demecolcine, a cytotoxic alkaloid used in chromosome analysis.
References in periodicals archive ?
Microscope slides were prepared after a standard 24- to 48-hour cell culture and harvested by using a 0.075M KCl hypotonic solution for 20 minutes at 37[degrees]C without colcemid followed by 3 changes, at room temperature, of freshly prepared 3:1 methanolglacial acetic acid fixative.
The addition of colchicine (or colcemid) pretreatment results in mitotic arrest and that treatment of arrested cells with a hypotonic solution like potassium chloride improved the yield and quality of metaphases spreads.
Cell cycling was arrested in metaphase by addition of 10 [micro]g/ml colcemid and incubating for 2-4 hours (Thermo Fisher Scientific[R], Paisley, UK).
Colcemid (Thermo Fisher, MA, USA), a cell cycle arresting agent, was used as a positive control at a concentration of 100 ng/ml.
Seis horas antes de la cosecha los medios fueron tratados con un pulso de BrdU y 1 hora antes con Colcemid. Transcurrido este tiempo se cosecho cada medio.
For this purpose, the cells were incubated for 2h and 30 min in the vicinity colcemid. KCL (0.075 M) was used as a hypotonic solution.
We have previously shown that oxidants such as hydrogen peroxide, menadione, and other chemicals including colcemid, amoxicillin, and flavonoids of propolis can inhibit gap-filling during the repair of UVC-induced DNA lesions [3].
At 46th hour of incubation, the cells were blocked at metaphase by adding colcemid at a final concentration of 0.1 [micro]g/mL and the cultures were further incubated until 48hrs.
Then, metaphases are harvested by adding colcemid (10 mg/l) for 120 min, followed by hypotonic KCl (0.075 M) treatment for 30 min, and fixation using stand 3:1 methanol-acetic acid.