of acetylation null CIITA mutants with pCAF.
The resulting cell lysates were subjected to overnight coimmunoprecipitation
(Co-IP) by using an anti-CREB antibody.
identification of protein interaction
Using the two-hybrid system, coimmunoprecipitation
and transfection studies on E2-dependent human breast cancer MCF-7 cells, it is herein reported that receptor for activated C kinase 1 (RACK-1), an intracellular G-protein-like molecule, [sup] is able to bind to and activate the human ER-a.
Importantly, ABT-737, a BH3-mimetic inhibitor that specifically targets BCL-2/ BCL-[X.sub.L], but not Mcl-1 [104, 105], disrupted the coimmunoprecipitation
of ATG12 with BCL-2, but not with Mcl-1 .
. Wild type Hepa1c1c7 cells were grown to 80% confluence.
In addition, nephrin forms complexes--as proven through coimmunoprecipitation
and pull-down assays--with cadherins, p120 catenin, and scaffolding proteins zonula occludens protein 1 (ZO-1), CD2AP, and calcium/calmodulin-dependent serine protein kinase (CASK) .
studies were conducted using [10.sup.6] BEAS-2B cells per experiment; cells were harvested in lysis buffer (1% NP40 detergent).
A few examples of specific topics include locomotor activity in rodents, automated video image analysis of larval zebrafish locomotor rhythms, identification of clock genes using difference gel electrophoresis, quantitative polymerase chain reaction, coimmunoprecipitation
assay, manipulation of mammalian cell lines for circadian studies, and immunocytochemistry on suprachiasmatic nucleus slices.
In order to elucidate dynamic biochemical interactions, an HA-tagged version of dbf4p was used in coimmunoprecipitation
experiments with cells blocked in early S phase as well as late mitosis.
In this study, we used coimmunoprecipitation
(co-IP) and employed mass spectrograph (MS) to find the candidate protein involved in AR trafficking.
Western Blot Analysis and Coimmunoprecipitation