cloning vector

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clon·ing vec·tor

an autonomously replicating plasmid or phage with regions that are not essential for its propagation in bacteria and into which foreign DNA can be inserted; this foreign DNA is replicated and propagated as if it were a normal component of the vector.
Farlex Partner Medical Dictionary © Farlex 2012
A recombinant DNA molecule containing sequences from 2 or more sources—e.g., plasmids, bacteriophage, cosmids—into which foreign DNA—40+ kb—may be inserted for cloning; a DNA sequence capable of self-replication in a suitable host cell, which has site(s) for inserting DNA fragments by recombinant DNA techniques, and genetic markers that allow selection for the vector in a host cell; CVs usually have a selective marker—e.g., for antibiotic resistance—and a site for cleavage by a restriction endonuclease
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.

clon·ing vec·tor

(klōn'ing vek'tŏr)
An autonomously replicating plasmid or phage with regions that are not essential for its propagation in bacteria and into which foreign DNA can be inserted; this foreign DNA is replicated and propagated as if it were a normal component of the vector.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

cloning vector

see VECTOR.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
Bernard, "New ccdB positive-selection cloning vectors with kanamycin or chloramphenicol selectable markers," Gene, vol.
"Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors".
The fragment of DNA was purified using 0.7% agarose electrophoresis gel and ligated into pCR(r)4-TOPO-TA cloning vector according to the manufacturer's instructions.
Two types of gene specific primers for each gene (Table 1) were designed based on cloning vectors used in cloning reactions of TaNAC69-1 and TtNAMB-2 genes in different expression vectors.
(1983) A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
asburiae MS7 followed by ligation of DNA fragments into the BamHI-site of cloning vector pGB2 (32).
The claims are directed to a modified glyphosate resistance gene, cloning vectors containing the gene, various plant cells containing the gene, and a process for inserting the gene into cells.
The amplified DNA was then prepared for insertion into cloning vectors by restriction with BarnHI and NdeI.
Scientists have been attempting to construct a variety of food-grade cloning vectors for use in lactic acid bacteria and to improve the bacteriophage resistance of dairy starter cultures using genetic engineering techniques.
In brief, the pGEM-T cloning vectors containing nFGH or oFGH gene fragments were digested with EcoRI and NotI restriction enzymes and the fragments with molecular weight of about 600 bp were purified using QIAquick Gel Extraction kit (Qiagen, USA).
Application of the knowledge generated from this fundamental genetic research is being implemented toward the construction of more sophisticated food-grade cloning vectors.
A fosmid is a cloning vector into which a foreign DNA fragments were inserted for cloning.