citrate synthase


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Related to citrate synthase: Succinate dehydrogenase, ATP citrate synthase

cit·rate syn·thase

citrate (si)-synthase; an enzyme catalyzing the condensation of oxaloacetate, water, and acetyl-CoA, forming citrate and coenzyme A; an important step in the tricarboxylic acid cycle.

citrate

any salt of citric acid. Citrate is the first intermediate of the citric acid cycle and tricarboxylic acid (TCA) cycle. It also plays an important role in fatty acid synthesis which takes place in the cytoplasm. It acts as a carrier of acetyl-CoA, the construction material for fatty acids. The movement is assisted by two enzymes, citrate-condensing enzyme, which catalyzes the condensation of the acetyl unit with oxaloacetate in the mitochondria, and citrate-cleavage enzyme (citrate lyase), which catalyzes the release of the acetyl radical in the cytoplasm of the cell.

citrate cleavage enzyme
important enzyme in fatty acid synthesis from glucose; catalyzes the split of citrate to acetyl CoA and oxaloacetate.
citrate synthase
first enzyme of the TCA cycle catalyzing the formation of citrate from acetyl CoA and oxaloacetate.
References in periodicals archive ?
In addition, citrate synthase activity was significantly higher in the training group, and the increase was also a function of the distance run (r = 0.
Intercorrelations among variables, excluding citrate synthase, also were high, suggesting that the inclusion of additional variables would not have significantly increased resolution in our analysis.
49), and citrate synthase were assayed by methods described elsewhere (17-21).
Final mitochondrial isolates were subjected to 3 freeze/thaw cycles and activity of respiratory chain enzyme complexes I + III and II + III and citrate synthase (CS) was measured spectrophotometrically as previously described [39] on a BioTek Synergy (VT, USA) H1 microplate reader.
Similar to the preparation of tissue homogenates, the mitochondrial suspension was preincubated for 10 min at 37[degrees]C before PDHc measurement, and an aliquot of the mitochondrial suspension was used for measurement of citrate synthase activity and protein content (21, 22).
DNA by using a PCR and primers CS443f and CS1210r specific for a 767-bp fragment of the citrate synthase gene (5).
All automated assays (including citrate synthase and protein) were performed at 37 [degrees]C on a Mira S automatic analyzer (Roche).
We used 2 Rickettsia-specific primer sets (CS2-78/CS2-323 and CS4-239/CS4-1069) to amplify 401 bp and 834 bp, respectively, of the citrate synthase gene (gltA) (5,6).
The activities of NADH dehydrogenase (complex I) (18), succinate dehydrogenase (complex II) (19), NADH cytochrome C reductase (complex I and III) (18), succinate cytochrome C reductase (complex II and III) (18), cytochrome C oxidase (complex IV) (20), and citrate synthase (CS) (21) were measured spectrophotometrically using a Beckman DU-650 Spectrophotometer by monitoring the reduction of ferricyanide (complex I), 2,6-dichlorophenolindophenol (complex II), cytochrome C (complex I+III and II+III), the oxidation of cytochrome C (complex W), and the appearance of free coenzyme A (CS) in spermatozoa homogenates as previously described.
1258n was selective for a 396-bp fragment of a highly conserved gene encoding the citrate synthase (gltA) shared by all Rickettsia spp.
Genes targeted by PCR included fragments of adenosine triphosphate synthase a subunit (atpA), heat-shock protein 70 (dnaK), outer membrane protein A (ompA), outer membrane protein B (ompB), citrate synthase (gltA), 16S rRNA, recA, and initiator protein of DNA replication (dnaA) (6,7).