In addition, citrate synthase
activity was significantly higher in the training group, and the increase was also a function of the distance run (r = 0.
Intercorrelations among variables, excluding citrate synthase
, also were high, suggesting that the inclusion of additional variables would not have significantly increased resolution in our analysis.
Mean citrate synthase
activities of mantle muscle ranged from 0.
49), and citrate synthase
were assayed by methods described elsewhere (17-21).
Final mitochondrial isolates were subjected to 3 freeze/thaw cycles and activity of respiratory chain enzyme complexes I + III and II + III and citrate synthase
(CS) was measured spectrophotometrically as previously described  on a BioTek Synergy (VT, USA) H1 microplate reader.
Similar to the preparation of tissue homogenates, the mitochondrial suspension was preincubated for 10 min at 37[degrees]C before PDHc measurement, and an aliquot of the mitochondrial suspension was used for measurement of citrate synthase
activity and protein content (21, 22).
DNA by using a PCR and primers CS443f and CS1210r specific for a 767-bp fragment of the citrate synthase
All automated assays (including citrate synthase
and protein) were performed at 37 [degrees]C on a Mira S automatic analyzer (Roche).
We used 2 Rickettsia-specific primer sets (CS2-78/CS2-323 and CS4-239/CS4-1069) to amplify 401 bp and 834 bp, respectively, of the citrate synthase
gene (gltA) (5,6).
The activities of NADH dehydrogenase (complex I) (18), succinate dehydrogenase (complex II) (19), NADH cytochrome C reductase (complex I and III) (18), succinate cytochrome C reductase (complex II and III) (18), cytochrome C oxidase (complex IV) (20), and citrate synthase
(CS) (21) were measured spectrophotometrically using a Beckman DU-650 Spectrophotometer by monitoring the reduction of ferricyanide (complex I), 2,6-dichlorophenolindophenol (complex II), cytochrome C (complex I+III and II+III), the oxidation of cytochrome C (complex W), and the appearance of free coenzyme A (CS) in spermatozoa homogenates as previously described.
1258n was selective for a 396-bp fragment of a highly conserved gene encoding the citrate synthase
(gltA) shared by all Rickettsia spp.
Genes targeted by PCR included fragments of adenosine triphosphate synthase a subunit (atpA), heat-shock protein 70 (dnaK), outer membrane protein A (ompA), outer membrane protein B (ompB), citrate synthase
(gltA), 16S rRNA, recA, and initiator protein of DNA replication (dnaA) (6,7).