chromosome walking


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chro·mo·some walk·ing

sequential isolation of overlapping sequences of DNA (that is, clones); with this procedure large regions of the chromosome can be spanned.

chromosome walking

A procedure for determining the nucleotide sequence of segments of DNA that are located on either side of a region of fully characterised (i.e., “sequenced”) DNA. Chromosome walking requires the dividing (“walking out”) of each segment of DNA into overlapping short (15–20,000) bases from a point of a known sequence; this enables one to sequence DNA far removed from a marker gene.

chro·mo·some walk·ing

(krō'mŏ-sōm wawk'ing)
A process of extending a genetic map by successive hybridization steps.

chromosome walking

a technique for mapping chromosomes that involves the sequential isolation of clones carrying overlapping NUCLEOTIDE SEQUENCES. This makes it possible to ‘walk’ along the chromosome from one gene to the next, to reach a desired LOCUS. Chromosome walking is used in genome sequencing projects, see CLONE CONTIG SEQUENCING METHOD.
References in periodicals archive ?
These data suggest markers Xfcg17 and Xfcg9 will provide excellent starting points for chromosome walking and the eventual map-based cloning of Tsn1.
(2003) showed that the availability of the extensive rice genome sequence data could be used to generate markers for saturation mapping to expedite chromosome walking to a gene of interest in a related species.
The central feature of map-based cloning is a technique called "chromosome walking." The scientists identified two marker genes, or sites of known traits, on the chromosomes of the disease-resistant tomato plant.

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