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The UV-visible absorption spectrum of cholic acid showed absorption band with maxima at 239 nm, while all metal cholates gave single band with maxima at 259.2 nm.
When copper, cobalt, and zinc cholate reacted with hot 1:1 HCl, the metal-cholic acid bond break ending with cholic acid and metal chloride mixture, which appeared as a faint precipitates, but chromium and nickel cholate did not respond to this reagent.
It worth mentioning that the only similar work found in literature is that of Kishu [6], who prepared a set of what they call organometallic complexes of cholic acid. Organometallic compounds should contain carbon-metal bond (C-M bond), metal cholates did not contain such bonds.
"Antibacterial activity of cholanic acids stereoisomers compared to cholic acid on the test cultures of microorganisms.", Annals of Mechnikov Institute, 2: 35-38.
Schillen et al., "Amino acid-bile acid based molecules: extremely narrow surfactant nanotubes formed by a phenylalanine-substituted cholic acid," Chemical Communications, vol.