Phylogenetic analysis of the sequences using the EzBiocloud classifier identified 14 phyla present in the Crassostrea gigas larvae and spat samples (Acidobacteria, Actinobacteria, Bacteroidetes, Calditrichaeota, Chlamydiae, Chloroflexi
, Chlorobi, Cyanobacteria, Firmicutes, Parcubacteria, Peregrinibacteria, Planctomycetes, Proteobacteria, and TM6).
Analisis metagenomicos de muestras de suelos contaminados con cianuro y derivados toxicos.--Los analisis metagenomicos de las muestras de suelos contaminados con cianuro mostraron 22 filos bacterianos identificados, predominando los filos Proteobacteria (12.91%), Firmicutes (11.32%), Bacteroidetes (10.16%), Actinobacteria (11.25%), Verrucomicrobia (8.20%) y con abundancias menores se encuentraron Chloroflexi
(0.73%), Synergistetes (0.59%), Thermodesulfobacteria (0.49%), Chlorobi (0.33%), Fibrobacteres (0.001%) y Gemmatimonadetes (0.001%); ademas, se reportaron tambien secuencias bacterianas no clasificadas, las cuales representan el 14.48% del total (Tabla 4).
was not obtained in sample C1, while it was identified in the other two samples.
Other dominant phyla identified in the gut of oysters, mussels, and clams include Tenericutes, Bacteroidetes, Actino-bacteria, Firmicutes, Chlamydiae, Fusobacteria, Spirochaetes, Chloroflexi
, Plantomycetes, and Verrucomicrobia (Fernandez-Piquer et al.
At the phylum level, bacterial communities in all samples were dominated by Firmicutes (relative abundance: 57.31% to 78.40%) and Proteobacteria (relative abundance: 31.02% to 16.94%), others were Bacteroidetes (relative abundance: 6.48% to 2.26%), Cyanobacteria (relative abundance: 6.97% to 1.23%), Actinobacteria (relative abundance: 1.14% to 1.98%), Chloroflexi
Of interest, there were two phyla, Chloroflexi
and Verrucomicrobia, in which OTUs were not related to other factors (presence of an indoor dog, home condition, or winter), but were significantly associated with current farming (Table 3).
Harding et al., "Natural niche for organohalide-respiring Chloroflexi
," Applied and Environmental Microbiology, vol.
, and Verrucomicrobia phyla were found in relatively low proportions less than 0.5%.
The proportion of other phyla (Acidobacteria and Chloroflexi
) was similar in the two soil types.
Based on these results, Proteobacteria, Chlorobi/Bacteroidetes, Actinobacteria, and Chloroflexi
have been identified as the most common bacterial classes.