As the second-most abundant biopolymer after cellulose, chitin is mainly synthesized via a biosynthetic process by an enormous number of living organisms such as shrimp, crab, tortoise, and insects and can also be synthesized by a nonbiosynthetic pathway through chitinase-catalyzed polymerization of a chitobiose
The average molecular weight of COS was 1,500 Da and the major components of COS were chitobiose
, chitotriose, chitotetraose, chitopentaose and chitohexaose (Li et al.
Because human N-linked glycans all contain the same chitobiose
core [2 N-acetyl-glucosamines (G1cNAcs) attached to a triman-nose] and are synthesized by a well-defined pathway, an accurate mass measurement will generally permit prediction of structure (23, 24).