Transient Gene Expression (TGE) is a well-established technology for rapid production of milligram to gram quantities of recombinant proteins in Human Embryonic Kidney (HEK) and Chinese Hamster Ovary (CHO) cell lines (1-3) Due to quick turnover and low cost, TGE platform plays more important role in bio pharmaceutical early development stages for two reasons; Firstly, it is essential for large biopharmaceutical companies to screen multiple drug candidates prior to moving forward into the formal development pipeline.
 evaluated the potential genotoxicity of pure 2,4-D (acid), and the commercially used salt in Chinese hamster ovary cells treated with 2.0-10.0 [micro]g/mL using SCE and single cell gel electrophoresis (SCGE) assays.
(1998) developed Super- CHO cell line (from Chinese Hamster Ovary (CHO) parent cell line) which expressed recombinant IGF-I that renders the cells to grow indifinitely in protein and/or serum-free media.
SAFC(R), a custom manufacturing and services business unit of Sigma-Aldrich Corporation (NASDAQ: SIAL), is introducing the CHOZN(R) Platform system, a CHO (Chinese Hamster Ovary) cell expression system.
Using their expertise in cell line, medium and feed development, raw material characterization, vendor qualification and supply chain management, the company is working to improve Chinese hamster ovary (CHO) monoclonal platform cell lines and to redesign the way the platform processes are conceived and executed, and the company's off-the-shelf platform will include parental CHO cells genetically engineered for enhanced bioproduction, and chemically defined media and feeds designed to work in harmony with the cell lines.