Chimeric Protein

A hybrid protein encoded by a nucleotide sequence spliced together from 2+ complete or partial genes produced by recombinant DNA technology
References in periodicals archive ?
Yurchenco, "Chimeric protein repair of laminin polymerization ameliorates muscular dystrophy phenotype," The Journal of Clinical Investigation, vol.
Advances in cytogenetics have revealed that the definitive diagnosis of synovial sarcoma can be made only by evidence of a translocation t(X:18) characterized by fusion of the SYT gene (Figure 5) on chromosome 18 with SSX genes on X chromosome resulting in a chimeric protein [5].
Chimeric protein 2 (CHN2) regulating Rac GTPase activity is the receptor for diglyceride and phorbol ester.
The chimeric protein might interfere the function of RANBP2, by competing for its ligands, acting as a dominant-negative effector, by blocking ligands from RANBP2 or by generating a misfolding of them.
The corresponding EML4ALK chimeric protein demonstrated constitutive tyrosine kinase activity that conferred transformation potential and responsiveness to specific targeted inhibitors in vitro and in vivo.
Expression of the chimeric protein was induced at [OD.sub.600] of 0.5 by addition of 1 mM isopropyl-[beta]-D-galactopyranoside (IPTG) and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Expression of a chimeric protein containing the catalytic domain of shiga-like toxin and human granulocyte macrophage colony-stimulating factor (Hgm-csf) IN Escherichia coli and its recognition by reciprocal antibodies.
Recent advances in molecular methodologies have allowed gene fusions and chimeric protein construction.
The ETV6-NTRK3 gene fusion encodes a chimeric protein tyrosine kinase that transforms NIH3T3 cells.
The expression system includes a protein expression vector that contains a chimeric gene encoding a chimeric protein. The chimeric protein contains three polypeptidyl fragments: (a) a first polypeptidyl fragment at the N-terminal end of the chimeric protein that contains a protein transduction domain (PTD) or a fragment thereof having HIV Tat PTD activity; (b) a second polypeptidyl fragment at the C-terminal end of the first polypeptidyl fragment that contains a J-domain or a fragment thereof having heat shock protein 70 (Hsp70)-interacting activity; and (c) a third polypeptidyl fragment at the C-terminal end of the second polypeptidyl fragment that contains a target protein or polypeptide.
This chimeric protein reveals what parts of GLUT4 play a role in indinavir's inhibition of glucose absorption.