However, an age-associated lack of neutrophil chemokinetic
accuracy in response to the chemotaxin fMLP has been reported , which suggests that the mechanism is probably related to age-induced compromise of specific cellular mechanisms, rather than activation.
potential of the cells decreased significantly, as did the levels of the ratio of ATP to ADP, if cells were starved at higher temperatures.
The CCL2 chemokine is efficient on OE-MSC migration in the presence of a chemokine gradient, suggesting that CCL2 effects are mainly chemotactic, whereas SPP1 induces similar responses in the presence or absence of a gradient, demonstrating that its effects are mainly chemokinetic
. Moreover, at the concentration showing the maximum efficacy for the two cytokines, CCL2 is more potent than SPP1 and a cocktail of both cytokines does not increase CCL2 effects.
Sherry et al., "Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic
properties," Journal of Experimental Medicine, vol.
In contrast, a density of M2-macrophages at the invasive front higher than in intraepithelial regions of the tumour leads to enhanced invasion through secretion of chemokinetic
growth factors that remodel the extracellular matrix, thus shortening patients' survival (Figures 2(b) and 2(c)).
Skopulska-Rozewska, "In vivo stimulatory effectof Multi-component herbal remedy PADMA 28 on mitogen-induced proliferation of mice splenic lymphocytes and their chemokinetic
activity," Polish Journal of Veterinary Sciences.
To exclude a solely chemokinetic effect of HGF on the migration of MSC, we simultaneously added the same concentration of HGF to the top and the bottom compartment and also only to the top compartment.
To check for any chemokinetic influences of HGF, the assay was carried out with the same concentration of HGF but differently localized (no HGF = basal migration, bottom compartment only, top and bottom compartment, Figure 5; and top compartment only, Figure 6).
To exclude any possible chemotactic or chemokinetic effect of Matrigel on the migration of MSC, the modified Boyden chamber assay was carried out without collagen and fibrin gel, but only with Matrigel on the bottom of the plate.