Checkerboard Assay

A type of Boyden chamber assay for leukocyte chemotaxis, first introduced by Zigmond
References in periodicals archive ?
Synergy between M/R and MeSNA was assessed using a microbroth dilution checkerboard assay and lowest fractional inhibitory concentration (FIC) analysis as described by the Clinical Microbiology Procedures Handbook [33].
Moreover, we have evaluated antifungal activity and synergistic effects of EOMS, with fluconazole and micafungin by NCCLS microdilution method, associated with the broth microdilution checkerboard assay.
Data from checkerboard assay were analysed and interpreted using the fractional inhibitory concentration index and the response surface approach using the [DELTA]E model.
The checkerboard assay was carried out as described by Lorian (2005) with slight modifications as stated in the broth microdilution method above (Lorian 2005).
Fractional inhibitory concentration (FIC) from checkerboard assay of ceftazidime plus clavulanic acid, apigenin.
aureus used in the checkerboard assay. Strain Median MIC ([mu] g/ml) (range) Antibiotic CLI ERY GEN LVX OXA ATCC43300 8192 (512-1024) (64-256) 256 [less (8-16) 8 1024 than or equal to] 0.5 AQ004 8192 (512-1024) (16-32) 32 (16-64) (128-256) 1024 16 128 AQ006 (64-128) 512 (128-256) 128 (8-32) 2048 128 8 AQ007 (32-64) 32 (16-64) 64 128 (16-64) (256-512) 16 256 AQ012 16384 (1024-2048) (32-64) 64 32 (256-512) 1024 512 Clindamycin, erythromycin, gentamicin, levofloxacin and oxacillin were chosen for the drug-interaction assay, since they belong to different classes of antimicrobial agents, respectively, lincosamides, macrolides, aminoglycosides, quinolones and [beta]-lactams.
The checkerboard assay described by Verma (2007) was followed with modifications.