chaotropic agent


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chaotropic agent

An ion that disrupts membranes, nucleic acids, and proteins.
See also: agent
References in periodicals archive ?
The manual specimen preparation method isolates CMV DNA from 200 [micro]L of plasma by lysis of the virus with a chaotropic agent, guanidine thiocyanate, followed by precipitation of the DNA with isopropyl alcohol.
Lytic enzymes, chaotropic agents, and different types of detergents are the main components of chemical lysis, while mechanical method disrupts the cells by grinding, shearing, bead beating, and shocking [8].
Given our observed inability to greatly alter soy flour adhesion using chaotropic agents, salts, surfactants, or co-solvents (Frihart and Lorenz 2013), which is in contrast to the literature observations with soy protein isolate (Sun 2005b), we concluded that the carbohydrates were suppressing protein alteration for improved adhesion.
Most human cells and other pathogens require only simple chemical lysis (alkaline lysis, salt, detergents, or chaotropic agents), especially viruses and gram-negative bacteria.
TEST PARAMETERS Inclusion Body Wash Solubilization Refold Buffer composition Buffer composition Buffer composition * Chaotropic agents * Chaotropic agents * Chaotropic agents * Detergents * Detergents * Redox agents * Salts * Reducing agents * Amino Acids * Detergents * Sugars * Salts w/v ratio Duration Duration Cycles of Protein Protein centrifugation concentration concentration pH w/v raio