cellomics


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cellomics

study of the CELLOME, to provide an understanding of all the processes that govern cell function.
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IGG members report experience with several of these systems, including Array Scan (Cellomics, Inc., Pittsburgh, PA), Noldus System DanioVision with the DanioScope (Noldus Information Technology Inc.
Following a career start as an analytical chemist at Smithkline French and Beckman Research, a predecessor company of GlaxoSmithKline, Kinnon went on to hold leadership positions with Transgenomic, ZyGEM, Life Technologies, Guava Technologies, and Cellomics.
Previously, Kinnon has worked in leadership positions with Transgenomic, ZyGEM Corp., Life Technologies, Guava Technologies as well as Cellomics.
NF-[kappa]B activity was measured in the macrophages using indirect immunofluorescence with a Cellomics NF-[kappa]B kit (Thermo Scientific) according to the manufacturer's protocols.
Cell images were acquired by using Thermo Fisher Scientific Cellomics ArrayScan VTI High Content Screening Reader and images were analyzed by using the Compartmental Analysis BioApplication (Thermo Fisher Scientific).
Digital images of each well were captured using a Cellomics ArrayScan VTI (Thermo Scientific Cellomics[TM]) (0.8 NA objective, 0.63x optical coupler, and XF-93 filter set) at 20x magnification.
Fluorescence was determined by using the Cellomics High Content Screening ArrayScan VTI imaging system (ThermoFisher Scientific, Waltham, MA, USA).
Cellomics Cell Cycle kit 1 (Thermo Scientific, Pittsburgh, PA) was used for analyzing the cell cycle distribution induced by the quinazolinone-based compounds.
The number of fluorescent nuclei was quantified using the CY3 fluorescence channel of the NIKON Cellomics ArrayScan VTI HCS Reader (Thermo Scientific).
This fluorescent dye stains only cells with compromised cell membranes thereby enabling their exclusion from NBD-PE cell quantitation by the Cellomics ArrayScan instrument.
"With the creation of the SCOC, we assemble a strong multidisciplinary consortium of academia and industry, and an interdisciplinary team of senior scientists, engineers and informatics specialists with very deep knowledge and skills in analyzing cellomics and genomics information.