Purification and characterization of a thermostable cellobiohydrolase
from Thermotoga petrophila.
Regarding cbh1 and cbh2, the two main cellobiohydrolases
, it has been demonstrated that growth of T.
thermophilum, such as a GH55 b-1,3-glucanase (Papageorgiou and Li, 2015), a b-glucosidase (Xu et al., 2011) and a cellobiohydrolase
II (Wang et al., 2013).
Harjunpaa et al., "Tryptophan 272: an essential determinant of crystalline cellulose degradation by Trichoderma reesei cellobiohydrolase
Cel6A," FEBS Letters, vol.
. -- Production of cellobiohydrolase
was induced in cultures with all carbon sources tested.
The activity of cellobiohydrolase
(CBH), an extracellular enzyme, was used as a measure of cellulose-degrading potential.
activity varied greatly during the whole period of decay and disappeared at the advanced stages of decay.
This activity was significantly higher (P = 0.05) than the cellobiohydrolase
activities evaluated in fluids from cultures containing microcystalline cellulose, CMC or xylan (Table 1).
There are three major groups of enzymes in cellulase system on the basis of their action: endo-[beta]-1, 4-glucanase or non-processive cellulases (EG, EC 184.108.40.206 alternatively called [C.sub.x], CMCase and endoglucanase or 1,4-[beta]-D-glucanohydrolase), exo-[beta]-1, 4-glucanase or processive cellulases (avicelase, exoglucanase, cellobiohydrolase
, [G.sub.1], CBH, EC 220.127.116.11) and [beta]-1, 4-glucosidase (BG, EC 18.104.22.168)
In this paper, we describe the production of recombinant cellobiohydrolase
II (CBHII, EC 22.214.171.124) from T.
Synergistic enhancement of cellobiohydrolase
performance on pretreated corn stover by addition of xylanase and esterase activities.
Cellulase is a multi-component enzyme comprising of endoglucanase (EC 126.96.36.199), which attack cellulose in amorphous zone and release oligomers, cellobiohydrolase
(EC 188.8.131.52), that liberate cellobiose from reducing and non-reducing ends and b-glucosidase (EC 184.108.40.206), which hydrolyze cellobiose to glucose and play a key role in avoiding cellobiose inhibition and thus enhancing the hydrolysis rates of cellulose into glucose (David, 2008; Mehdi et al., 2010; Sunkyu et al., 2010; Baljit et al., 2014; Veeresh and Wu, 2014).