cellobiase


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β-d-glu·co·si·dase

(glū-kō'si-dās),
A glucohydrolase similar to α-d-glucosidase, but attacking β-glucosides and releasing β-d-glucose.
References in periodicals archive ?
Activity of cellobiase was determined at different pH ranging from 5.8 to 8.0.
After anion exchange chromatography on DEAE cellulose column two different cellobiase isoforms named C-I and C-II were identified with purification folds of 21.16 and 31.83, respectively.
Lau and Wong (2001) purified a novel cellobiase Cba 2 from Cellulomonas biazotea over 260 fold using ammonium sulphate precipitation, gel filtration chromatography, anion-exchange chromatography and reversed phase high performance liquid chromatography sequentially.
Effect of temperature on partially purified cellobiase
Here, Cellobiase activities were measured in partially purified cellobiase at different temperatures ranging from 5 to 35[degrees]C.
Roy et al (2005) reported that biological activities of urea denatured forms of carboxymethylcellulase, cellobiase and [beta]-glucosidase can be recovered using three phase partitioning using [(N[H.sub.4]).sub.2]S[O.sub.4] and t-butanol.
CMCase, Cellobiase and FilterPaperase were produced and partially purified by ammonium sulphate precipitation (0-30 and 30-80%) and dialysis followed by ion exchange chromatography using DEAE-cellulose column.
0.5 g salicin in 100 ml 0.2 M phosphate buffer solution) for cellobiase, respectively.
Increased level of dietary rice straw decreased CMCase ([P.sub.linear effect] = 0.02), xylanase ([P.sub.linear effect] <0.01) and cellobiase ([P.sub.linear effect] <0.01) activities, but increased the avicelase ([P.sub.linear effect] <0.01) activity (Table 4).
Although increased avicelase enzyme was observed, the other three fibrolytic enzymes, CMCase, xylanase and cellobiase, were decreased.
In addition, analysis of fibrolytic enzymes indicated that increasing level of dietary rice straw decreased the ruminal CMCase, xylanase and cellobiase activities, and increased ruminal avicelase activity.
Effect of increasing level of rice straw on activity of avicelase, CMCase, xylanase and cellobiase (n = 4) (1) Diets (2) Item RS5 RS10 RS15 RS20 Avicelase 5.59 b 6.38 a 6.09 a 6.33 a CMCase 3.96 a 3.13 b 3.77 a 3.30 b Xylanase 14.7 a 13.8 b 12.4 c 13.4 b Cellobiase 3.83 a 3.07 b 3.09 b 3.12 b Effects Item SEM Linear Quadratic Avicelase 0.18 0.02 0.09 CMCase 0.11 <0.01 0.03 Xylanase 0.21 <0.01 <0.01 Cellobiase 0.08 <0.01 <0.01 (1) Means with different letters within a row are significantly different (p < 0.05); SEM is pooled standard error of means.