catechol 2,3-dioxygenase

cat·e·chol 2,3-di·ox·y·gen·ase

(kat'ĕ-kol dē-oks'ē-jen'ās),
An oxidoreductase oxidizing catechol, with O2, to 2-hydroxymuconate semialdehyde.
Synonym(s): metapyrocatechase
References in periodicals archive ?
C23O is catechol 2,3-dioxygenase that catalyzes ring cleavage of the catecholic compounds in Gram-positive Planococcus sp.
In one of those mutant form of catechol 2,3-dioxygenase TGC codon for cysteine at position 103 had been changed to a CGC codon for arginine and the AAG codon at position 289 for lysine had been changed to a UAG stop codon [22].
Amino acid sequence of mutated form and the wild-type catechol 2,3-dioxygenase was deduced using the CLC Free Workbench 4.0.1 software.
In order to determine catechol 2,3-dioxygenase activity, the formation of 2-hydroxymuconic semialdehyde (2-HMS) was measured at 375 nm ([[epsilon].sub.2-HMS at 375 nm] = 36,000/M cm) in a reaction mixture containing 20 [micro]L of catechol (50 mM), 960 [micro]L of phosphate buffer pH 7.5 (50 mM), and 20 [micro]L of crude extract in a total volume of 1mL [26].
The substrate specificity of mutated and wild-type catechol 2,3-dioxygenase was examined with 3-methylcatechol, 4-methylcatechol, and 4chlorocatechol.
Structural Properties of the Wild-Type and Mutant Catechol 2,3-Dioxygenase. Amino acid sequence in the polypeptide chain defines protein structure and properties.
Effect of pH on the Mutant Catechol 2,3-Dioxygenase. To verify if introduced mutations influenced C23O activity, the effect of pH on the mutated and wild-type enzyme activity was determined.
di Donato, "The role of the conserved residues His-246, His-199, and Tyr-255 in the catalysis of catechol 2,3-dioxygenase from Pseudomonas stutzeri OX1," The Journal of Biological Chemistry, vol.
Fujisawa et al., "An archetypical extradiol-cleaving catecholic dioxygenase: the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase) from Pseudomonas putida mt-2," Structure, vol.
Zylstra, "Comparative analysis of the catechol 2,3-dioxygenase gene locus in thermoacidophilic Archaeon Sulfolobus solfataricus strain 98/2," Biochemical and Biophysical Research Communications, vol.
Guzik, "Properties of catechol 2,3-dioxygenase from crude extract of Stenotrophomonas maltophilia strain KB2 immobilized in calcium alginate hydrogels," Biochemical Engineering Journal, vol.
Kim, "Characteristics of catechol 2,3-dioxygenase produced by 4-chlorobenzoate-degrading Pseudomonas sp.