In contrast to T47D cell lines, MCF7 cell lines with tiliroside of 50 [micro]g/ml occurred weak expression of Caspase 8 (Figure 6) moreover Caspase 9 was not expressed.
These results have a similar profile for Caspase 9, in which tiliroside activates Caspase 9 and doxorubicin did not activate it.
This is through the release of Cytochrome c from mitochondria is a central event in the death receptor-independent, "intrinsic," apoptotic pathway which facilitates activation by Caspase 9
of the effector Caspases , which subsequently activates Caspase 3, and ultimately leads to the apoptotic cell death.
8 and Caspase 9 colorimetric assay: R&D Systems, Inc.
(California, USA) were used consisting of [beta]-Actin (1:1000), Bax (1:1000), Bcl2 (1:1000), Hsp70 (1:1000), survivin (1:1000), caspase 3 (1:1000) and caspase 9 (1:1000).
Initiating caspase 9
plays a role in the intrinsic pathway of the apoptosis, but in the extrinsic pathway the key role is played by the death receptor and by caspase 8 [41-43].
Methods: The cytotoxicity of the compounds was determined using a resazurin reduction assay, whereas the caspase-Glo assay was used to detect the activation of caspases 3/7, caspase 8 and caspase 9
in cells treated with compounds 3.
Effect of GS-Rb1 on the Activation of Caspase 3 and Caspase 9
. Activation of caspase-3 and caspase-9 is a key irreversible point in mitochondria mediated apoptosis.
The caspase 9
antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Compounds 2-4 strongly induced apoptosis in CCRF-CEM cells via caspases 3/7, caspase 8 and caspase 9
activation and disruption of MMP.
High levels of caspase-8 caspase 9
were also detected.
Effect of becatamide on caspase 9
activity in [H.sub.2][O.sub.2]-treated PC12 cells