ZN staining (4): Smears were flooded with filtered 1% carbol fuchsin
(CF) and heated until they were steamed and left to steam for 5 min.
Smears were prepared from all positive stool samples and stained separately with malachite green, methylene blue, crystal violet, and carbol fuchsin
Covered the slide with carbol fuchsin
it colorized all the ingredients in smear.
The fixed smears of (either wild caught or Wolbachia cured) were flooded with freshly filtered carbol fuchsin
solution prepared from 2 ml of stock solution of basic fuchsin in 5 ml of phosphate buffer for 10 sec.
And the meristematic tissue of root tips (about 2 mm length of the root tips) were dyed by carbol fuchsin
and used for cytological observation.
a thin film (smear) of fresh faeces on a clean glass slide was prepared and dried at room temperature in the field and was then examined by microscopy after conducting a Kinyoun's carbol fuchsin
modified acid-fast stain.
The fixed root tips of 80 regenerants were squashed and stained with carbol fuchsin
according to the method described by Jauhar et al.
They were stained with Kinyoun's carbol fuchsin
modified acid-fast stain and examined by oil immersion microscopy.
In 1883, German pathologist Friedrich Neelsen added his modification, using carbol fuchsin
instead of carbol methyl violet and decolorizing with 15% sulfuric acid.
Five-micrometer-thick sections were deparaffinized in xylene taken stepwise to water and stained in filtered carbol fuchsin
for 1-3 h at 56[degrees]C.
The smear is covered with strong carbol fuchsin
and gently heated to steaming for 5-7 min without letting the stain boil and become dry.
Later, all sandflies were stained with 20% carbol fuchsin
solution and then identified to species by microscope-based on some typical morphologic characteristics: mainly internal structures (such as hair on abdominal tergites, buccal capsule, pigment patch, pharyngeal basket and spermatheca, ciborium, pharynx for females and terminal genitalia for males).