Because 3' UTR sequences of porcine CYP19A1 and FSHR genes have not been reported, we aligned the 3' flanking sequences of these two genes with the respective human 3' UTR sequences to get the consensus sequences for further prediction.
However, no differences were detected in the methylation levels on BMP4, PCNA, or CYP19A1 gene promoters between SP and LP neonatal piglets ovary.
Expression of miRNAs predicted to target CYP19A1, FSHR, BMP4, and PCNA
We found that the expression of miR-423-5p targeting CYP19A1 and PCNA, miR-378 targeting CYP19A1 and miR-210 targeting BMP4 were significantly down-regulated (p < 0.
In addition, there is no difference in the DNA methylation of PCNA, BMP4, and CYP19A1 promoter.
However, we detected the miRNA regulation, and found that the expression of miR-423-5p and miR-378 targeting CYP19A1 was significantly downregulated, implying that the post-transcriptional repression of CYP19A1 may be decreased which thereby results in higher CYP19A1 protein content or enzyme activity.
Elevated level of 17P-estradiol is associated with overexpression of FSHR, CYP19A1, and CTNNB1 genes in porcine ovarian follicles after prenatal and neonatal flutamide exposure.
We used the PCR to analyze these 2 CYP19A1 polymorphisms in the 3' UTR (Fig.
In addition, we genotyped a series of 40 individuals for both CTLA4 and CYP19A1 polymorphisms by H-ARMS-PCR analysis with different thermal cyclers (iCycler[R] by Bio-Rad, Px2[R] by Hybaid, and PTC-100[R] by MJ Research) and in different laboratories, and we obtained fully concordant results, demonstrating the robustness and reproducibility of this approach.