Ulteriorly, fifteen module-related DEGs were obtained according to PPI network and modular analyses with a score no less than 4, namely, CXCL2, CXCL1, BDKRB1, LPAR1, CXCL3
, FYN, COL6A2, COL18A1, COL13A1, COL5A1, TUBA1A, FDFT1, SQLE, LSS, and CDK9.
Target Primer Sequence (5'-3') TNF-[alpha] F TTCTGTCTACTGAACTTCGGGGTGATCGGTCC R GTATGAGATAGCAAATCGGCTGACGGTGTGGG IFN-[gamma] F ACTGGCAAAAGGATGGTGAC R TGAGCTCATTGAATGCTTGG IL-6 F AACGATGATGCACTTGCAGA R GAGCATTGGAAATTGGGGTA MCP-1 F CCCACTCACCTGCTGCTACT R TCTGGACCCATTCCTTCTTG Cxcl3
F AGGCTACAGGGGCTGTTGT R GGGTTGAGGCAAACTTCTTG IR F GAGATGGTCCACCTGAAGGA R GGACAGACATCCCCACATTC IRS-1 F AAGCACCTGGTGGCTCTCTA R TCAGGATAACCTGCCAGACC IRS-2 F ATACCGCCTATGCCTGTCTG R TGGTCTCATGGATGTTCTGC GAPDH F AGTCCATGCCATCACTGCCACC R CCAGTGAGCTTCCCGTTCAGC Table 2: Calorie and composition of Seyoeum.
A specific primer for CXCL3
, NME7 and C5 is as follows: CXCL3
: FW: GGCTTTCCAGTCTCAACCAT, REV: GGCGGGACTTACATGACTTC; NME7: FW: GTCCTGACCAACCTCTTGGA, REV: GCAGTACCCCATAGACTGGTG; C5: FW: TTTCAGGACTGCTTGTAGGTGA, REV: AACTGGAGGAATGGAGTTTCC.
As obviously shown in Figure 7(a), the mRNA expression of CXCL3
, ELF5, and TIMP1 significantly increased in CRC tissues compared with matched adjacent normal tissues, whereas PHLPP2 mRNA expression was decreased in CRC tissues; and these results were further confirmed by TCGA database (Figure 7(b)).
In comparison to wt mice, TCDD-treated AhRR Tg mice had significantly lower expression of CXCL2 and CXCL3
Additionally there also were genes related to the CXC family of chemokines (MCP-1, IL-8, CXCL1, CXCL2, CXCL3
, and CXCR4).
We next matched genes in these three pathways with the 163 genes and identified 12 candidate genes: B-cell CLL/lymphoma 6 (BCL6), IL1[alpha] (ILIA), IL8, PTGS2, DTR, chemokine (C-X-C motif) ligand 3 (CXCL3
), promyelocytic leukemia (PML), sine oculis homeobox homolog 1 (Drosophila) (SIX1), tumor necrosis factor (TNF), [alpha]-induced protein 3 (TNFAIP3), Zn finger and BTB domain containing 1 (ZBTB1), Zn finger protein 44 (KOX 7) (ZNF44), and Zn finger protein 450 (ZNF450).
In castration-resistant prostate cancer from the xenograft mice, release of IL-1[alpha] from the necrotic primary cancer cells results in CXCL3
secretion, which, in turn, recruits B cells.
The ELR-motif-positive CXC chemokines, CXCL1, CXCL2, CXCL3
, CXCL5, CXCL6, CXCL7, and CXCL8, directly promote the migration and proliferation of endothelial cells and eventually neovascularization, mainly by interacting with CXCR2 but not with CXCR1 .
Angiogenic chemokines, such as CXCL1, CXCL2, CXCL3
, CXCL5, CXCL6, and CXCL8, are generally distinguished by an ELR motif.
Additional chemokines are upregulated by LOX-1 activation in response to OxLDL, including IL-8, chemokine (CX-C motif) ligands 2 and 3 (CXCL2 and CXCL3
OxLDL administration was also shown to induce the production of IL-6 from HUVECs , chemokines CXCL2 and CXCL3
from LOX-1 overexpressing human aortic endothelial cell line , MCP-1 from human articular chondrocytes , and the intestinal tissue of animals with endotoxemia .