Cx36, together with a site-specific expression of Cx45
and Cx57, is the principal connexin expressed in neurons.
Additionally, the effect of Wenxin Granule on Cx43 and Cx45, those involved in gap junction pathway, was also observed in the present study.
Cx45 forward: 5'-GGGCTCTGGAAGAAACGG AA-3'; reverse: 5' -ATGCTTGGGTTTTGGTTGGC-3'.
Expression of Connexin 43 (Cx43) and Connexin 45 (Cx45) Proteins.
In addition to Cx43, another gap junction protein, Cx45, although present at relatively low levels in the working myocardium, is absolutely required to maintain cardiac rhythms .
To date, at least 15 connexin genes have been reported in mammals, and seven genes (Cx26, Cx30, Cx32, Cx37, Cx43, Cx45
, and Cx60) are expressed in the ovary.
Importantly, transfer of cGAMP occurs through GJCs between Cx43 and Cx45
overexpressing cells .
In fact, Cx45
has been shown previously to be ex pressed by rat ovarian GCs (82).
In our recent study, we investigated the expression of selected connexins such as Cx36, Cx37, Cx40, and Cx43 mRNA by RT-qPCR as well as distribution of Cx30, Cx31, Cx37, Cx43, and Cx45
proteins in porcine GCs during short-term (168 h), real-time cell proliferation in vitro .
Cells were incubated with primary antibodies against desmin, myogenin (Abcam, Cambridge, UK), Cx43 (Transduction Laboratories, Lexington, KY, USA), or Cx45
(Invitrogen, CA, USA) for 1 h or with Alexa Fluor 594 phalloidin (Invitrogen, CA, USA) for 30 min at 37[degrees]C.
The most common isoforms in mammals are Cx36, Cx43,and Cx45
. Connexins are traditionally described as being expressed in astrocytes and in neurons.
Microglia express low to undetectable levels of Cx32, Cx36, Cx43, and Cx45