After sedimentation for 12 hours, part of the sediment was placed on glass slide, added lugol and examined under light microscopy (Olympus CX40
; objectives: 10x, 40x, and 100x), being used a 40x increase for the photographs.
Instruments include the full-automatic tissue dehydrator (Tianjin Aihua Medical Instrument Co., Ltd.), slicing machine (German SLEE), CX40
optical microscope (Japan Olympus), DYY-6C electrophoresis apparatus (Bejing Liuyi Instruments Co., Ltd.) and DYYm40B water-bath electrophoresis transfer tank (Beijing Liuyi Instruments Co., Ltd.).
Cells were counted in five squares on both sides using a microscope (Olympus CX40
different connexin types (Cx43, Cx40
, Cx37) and are located at the
Finally, samples were analyzed by means of a fluorescence microscope (CX40
, Olympus, Milan, Italy).
The appearance of the alginate microbeads and microcapsules obtained using the vibrating technique was assessed using an Olympus CX40
optical microscope, at 60x magnification.
) and Gjd3 (Cx31.9) were downregulated in GK compared to control SAN.
The expression regions of Cx40
, Cx37, and Cx43 are mainly restricted on the cytomembrane of vascular ECs .
The slides were stained using the Papanicolaou method and assessed using the valid cytological slide screening method according to the Bethesda system with the Olympus CX40
Desplantez, "Cardiac Cx43, Cx40
and Cx45 co-assembling: involvement of connexins epitopes in formation of hemichannels and gap junction channels," BMC Cell Biology, vol.
Subsequently, the cells became round and exhibited lipid droplets, as observed under an Olympus CX40
microscope (Center Valley, PA, USA) at 200 x magnification.