For microscopic examinations, free-hand sections of ascomata were mounted in water, 5% KOH, 1% phloxine or cotton blue, and observed under light microscope (Olympus
CX31).
Las observaciones microscopicas se realizaron en un microscopico de luz Olympus
CX31 y DMS 653 (sistema digital), montando las muestras en KOH al 5%, rojo Congo al 1% y azul de lactofenol al 1%.
Smears were stained with rapid panopticus and analyzed under an optical microscope (Olympus
CX31).
All of the sections were evaluated using a Olympus[R]
CX31 microscope with a Moticam[R] 480 camera.
The identification of the galling insects was done to the lowest possible taxonomic level through identification keys (White & Hodkinson, 1985; Stehr, 1987; Burckhardt & Basset, 2000; Nieves-Aldrey & Blas, 2015) and in comparison with reference material, using compound (Olympus
CX31) and stereoscopic (Zeiss, Stemi dV4) microscopes.
Images were obtained with an Olympus
CX31 light microscope attached to image capture system (Olympus, Tokyo, Japan).
Las muestras de liquenes recolectadas y etiquetadas se secaron por exposicion al ambiente y, posteriormente, se llevaron al herbario forestal Gilberto Emilio Mahecha Vega (Seccion Criptogamas) UDBC, donde se realizo el correspondiente proceso de determinacion mediante claves taxonomicas (Coca y Sanin, 2010; Lucking, Aptroot, Chaves, Sipman y Umana, 2008; Moncada y Forero, 2006; Moncada, 2012) y la aplicacion de pruebas quimicas con NaClO, KOH, KI y parafenilendiamina, ademas del uso de esterescopio Leica TS1232W26 y microscopio Olympus
CX31 con los cuales se realzo la observacion y el corte de las estructuras reproductivas de los liquenes para la observacion de caracteres utiles en la identificacion taxonomica.
El analisis microscopico se realizo con un microscopio de luz Olympus
CX31 y DMS 653 (sistema digital) donde las muestras se acompanaron de KOH al 5%, rojo Congo al 1% y azul de lactofenol (13,15,41).
Cat's
CX31 transmission has been added to the 730 and 730 EJ, as well as the 735.
For the purpose of the multicenter study, ZN slides were examined with the bright field microscopy routinely used in each laboratory at 1000x magnification, whereas fm slides were examined with Olympus
CX31 microscopes with a TK-LED illumination (Tolket, Argentina), using 200x magnification for screening and 400x magnification for confirming and quantifying the slides.
Very small drop of each sample was placed on a microscope slide and the pictures were taken using a microscope (Olympus
CX31, Japan) at 100x and 400x magnification.
Gja5 (Cx40) and Gjd3 (
Cx31.9) were downregulated in GK compared to control SAN.
