For microscopic examinations, free-hand sections of ascomata were mounted in water, 5% KOH, 1% phloxine or cotton blue, and observed under light microscope (Olympus CX31
Las observaciones microscopicas se realizaron en un microscopico de luz Olympus CX31
y DMS 653 (sistema digital), montando las muestras en KOH al 5%, rojo Congo al 1% y azul de lactofenol al 1%.
Smears were stained with rapid panopticus and analyzed under an optical microscope (Olympus CX31
All of the sections were evaluated using a Olympus[R] CX31
microscope with a Moticam[R] 480 camera.
The identification of the galling insects was done to the lowest possible taxonomic level through identification keys (White & Hodkinson, 1985; Stehr, 1987; Burckhardt & Basset, 2000; Nieves-Aldrey & Blas, 2015) and in comparison with reference material, using compound (Olympus CX31
) and stereoscopic (Zeiss, Stemi dV4) microscopes.
Images were obtained with an Olympus CX31
light microscope attached to image capture system (Olympus, Tokyo, Japan).
transmission has been added to the 730 and 730 EJ, as well as the 735.
Very small drop of each sample was placed on a microscope slide and the pictures were taken using a microscope (Olympus CX31
, Japan) at 100x and 400x magnification.
Gja5 (Cx40) and Gjd3 (Cx31
.9) were downregulated in GK compared to control SAN.