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It was mixed with 100 [micro]l of 5M NaCl and 80 [micro]l of CTAB/NaCl (Sigma, USA) (20% CTAB in 2.
Total genomic DNA was extracted and purified from leaf tissue of 2- to 3-week-old greenhouse grown plants of all genotypes using the CTAB method.
Genomic DNA will be extracted via a modified CTAB method.
Ionic strength was maintained constant with NaCl for the reaction in absence of CTAB and showed an increasing effect in presence of CTAB.
9 ml 2x CTAB extraction buffer [100 mM Tris-HCl, pH 8.
Adsorption of CTAB onto perlite samples from aqueous solutions.
After that, 1/5 volume of 5 M NaCl and 1/10 volume of 10% CTAB were added to the supernatant and incubated at 65[degrees]C for 10 min.
Our previous study indicates that the presence of a small amount of CTAB (cetyltrimethyl ammonium bromide) is important for nanoplate formation (Wang et al.
CTAB is also needed for optimal protein activity from the proteins in the eosinophil.
The college's Geology Engineering Technology, Mining Engineering Technology, Electronics Engineering Technology and Instrumentation Engineering Technology programs have also achieved national accreditation from CTAB.
Addition of the cationic surfactant CTAB resulted in a 6-fold increase in polymer fluorescence intensity and a narrowed, blue-shifted polymer absorption spectrum, suggesting that interaction with surfactant helped to break up polymer aggregates.
For the separation of organic acids, 20 mM benzoic acid buffer with 4 mM CTAB was used as an electrolyte and the pH was adjusted to 5.
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