Since ATMSCs cultured in PCM did not express the nPC markers under study, we assessed the expression of genes that code for CD271, CD34, and NG2 (NGFR, CD34, and CSPG4, resp.) after coculture of ATMSCs with murine ECs (endothelial cell line EOMA) on Matrigel[R] (Supplementary Materials).
Pilot experiments demonstrated increased expression of CSPG4, NGFR, and CD34 (genes that code for NG2, CD271, and CD34, resp.) in ATMSCs that were cocultured with murine ECs on Matrigel (Supplementary Table 3).
Supplementary Table 2: real-time PCR gene expression analysis of NGFR, CSPG4, and CD34 in ATMSCs, EOMA cells, or ATMSCs cocultured with EOMA cells in 2D or on Matrigel.
In more recent work, Alcantara Llaguno et al (88) used the Ascl1 promoter and the Cspg4
(encoding NG2) promoter as drivers for cre-ER to further specifically target oncogenic alterations to both committed neural progenitor cells and adult OPCs, and adult OPCs only, respectively.
In tumors, placental-like CS chains are linked to a limited repertoire of cancer-associated proteoglycans including CD44 and CSPG4. The rVAR2 protein localizes to tumors in vivo and rVAR2 fused to diphtheria toxin or conjugated to hemiasterlin compounds strongly inhibits in vivo tumor cell growth and metastasis.
The chrondroitin sulfate proteoglycan (CSPG4) regulates human trophoblast function.
Seven proteins (Rho-related BTB domain containing protein 3 (RhoBTB3), zinc finger and BTB domain containing protein 44 (ZTBT44), obscurin isoform B, BAI1, RNA exonuclease 1 homolog, death-associated protein kinase 3 (DAPK3), and chondroitin sulfate proteoglycan 4 protein (CSPG4)) showed restricted expression in 1 to 3 groups.
DAPK3 and CSPG4 were both highly expressed in control and greatly reduced or absent in the patient groups.
Gene Fold upregulation Significance (all time points) FRZB 256.9 *** OMD 72.4 *** PDGFRL 55.7 *** LEPR 29.2 *** IGF2 22.6 *** NGFR 19.6 * MSX1 10.4 ** ANGPTL4 8.2 *** MT2A 7.7 *** SFRP1 7.3 *** TGFBR2 5.7 *** TGFBR3 5.7 *** SPP1 5.5 *** ALPL 4.9 *** SORT1 4.4 *** DVL2 3.2 *** PDGFRA 3.2 *** BMPER 2.8 ** S1PR1 2.8 *** FZD1 2.7 *** CSPG4
2.6 *** FZD4 2.5 *** NGFRAP1 2.4 *** FGFR1 2.1 *** SOX9 0.5 ** NES 0.4 ** VEGFA 0.3 *** SFRP4 0.2 ** Only genes with significant >2-fold induction: * p < 0.05, ** p < 0.01, *** p < 0.001, and *** p < 0.0001.
COL1A1 Alpha 1 subund of type I collagen ANKTMI Ankyrh-like protein FMOD Fibromodulin MGC3047 Containing two Immunogbbulin (Ig) domains PCDH17 Containing five cadherin domains DKFZP564L0862 Similar to ankyrh repeat (Asb1) LTBP7 Latent transforming growth factor beta binding prod LUM Lumican CSPG4
Chondrodin suffate proteoglycan 4 Cell signaling 1964086 (IMP-1) IGF-II mRNA-binding protein 1 1_931758 Similar to IGFALS SYCP2 Synaptonemal complex protein 2 MGC26655 Similar to RaP2 interacting protein 8, Ras pathway RGS7 Regulator of G protein sgnaling 7, G protein pathway KCNJ3 Signal Tronsduction/G protein pathway GFRA7 GDNF family receptor alpha 1 PPARGCI Peroxisome prolif.