This enzyme, in turn, blocked another protein called collapsin response mediator protein 2, CRMP2
The researchers further determined that edonerpic maleate works by binding to an intracellular protein called collapsin-response-mediator-protein 2 (CRMP2
), which is related to synaptic plasticity.
The researchers wondered if it might be possible to introduce a drug into the brain that would bind with CRMP2
and help it do its rewiring job better.
Second, LCM binds to collapsin response mediator protein2 (CRMP2
), which enhances the drug's antiepileptic activity.
Dihydropyrimidinase-related protein 2/collapsin response mediator protein 2 (DPYL2/ CRMP2
) binds to tubulin heterodimers to promote microtubule formation and stability, resulting in axonal growth and neuronal polarity .
and CRMP4 were highly expressed in the retinal ganglion cell layer when retinal axons were crossing the chiasm and approaching the tectum in zebrafish.
Background: Collapsin response mediator protein-2 (CRMP2
) has been shown to be involved in ischemia/hypoxia (IH) injury.
Extensive lists of GSK-3 substrates or GSK-3 binding proteins have been reported and include amyloid precursor protein, APC, ATP-citrate lyase, axin, axil, [beta]-catenin, c-jun, Jun B, Jun D, Ci155, C/EBP alpha, CRMP2
, CRMP4, CREB, CTP, cyclin D1, dystrophin, eIF2B, glycogen synthase, glucocorticoid receptor, heat shock factor 1, hnRNP, K-casein, KRP, MAB 1B, MAP 2, MAP 2C, MITF, c-Myc, L-Myc, alpha NAC nascent polypeptide-associated complex, NCAM, NDRG1, NDRG2, neurofilament L, neurofilament M, neurofilament H, Notch 1C, p21 CIP1, p53, presenilin, pyruvate DH, PP1 G-subunit, protein phosphatase inhibitor 2, stathmin, synphilin-1, RSK1, and Tau (https://thebiogrid.org/ and http:// www.genecards.org/).
Louis, MO, USA); goat anti-rat Nogo-A polyclonal antibody (1:1000, Novusbio, Littleton, CO, USA); rabbit antirat NgR1 polyclonal antibody (1: 1000, Abcam, Cambridge, UK); rabbit anti-rat RhoA monoclonal antibody (1: 1000, Cell Signaling Technology, Boston, MA, USA); rabbit anti-rat ROCK monoclonal antibody (1:1000, Cell Signaling Technology); rabbit anti-rat collapsin response mediator protein 2 (CRMP2
) monoclonal antibody (1:1000, Cell Signaling Technology, Boston, MA, USA); rabbit anti-rat pCRMP2 monoclonal antibody (1 : 1000, Cell Signaling Technology).
Yang et al., "The suppression of CRMP2
expression by Bone Morphogenetic Protein (BMP)-SMAD gradient signaling controls multiple stages of neuronal development," The Journal of Biological Chemistry, vol.
In addition, ROCK2 can phosphorylate collapsin response mediator protein 2 (CRMP2
), another microtubule associated protein, to induce growth cone collapse .
Dr Calum Sutherland and his team at Dundee University found the protein, called CRMP2
, plays an important role in the development of nerve tangles seen in the disease.