CRABP2

(redirected from CRABP-II)

CRABP2

A gene on chromosome 1q21.3 that encodes a member of the retinoic acid- and lipocalin/cytosolic fatty-acid-binding protein families which shuttles its cognate ligands from the cytosol to the nucleus. It is involved in the retinoid signalling pathway and is associated with increased circulating low-density lipoprotein cholesterol (LDL-C).
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CRABP-I is expressed in almost all types of cells, whereas CRABP-II is expressed mainly in the skin.
101] showed that expression of CRABP-II, but not CRABP-I, significantly induced RAR-mediated transcriptional activation of a reporter gene, indicating that CRABP-II indeed may be involved in transcriptional activity of ATRA.
Increasing levels of CRABP-II were shown in normal and leukemia cells of APL patients undergoing ATRA treatment [57].
To investigate a possible role for CRABP-II in bovine adipogenesis, we have cloned bovine CRABP-II cDNA and the coding region for CRABP-I.
CRABP-I is expressed ubiquitously in the tissues of adult animals, whereas CRABP-II appears to be limited to the skin (Ong et al.
PCR was performed with forward (5-YGCYGCC ACYATGCCNAACTTYTCTGGCAA-3, TaKaRa, Korea) and reverse primers (5'-CKCTCACTCTCGGACGTA GACCCTTGGTGCA-3', TaKaRa, Korea) designed as degenerative primers using an amino-acid sequence specific for homologs of the human and mouse CRABP-II (GenBank accession no.
To determine the tissue expression of bovine CRABP-II mRNA, total RNA was extracted from heart, liver, lung, colon, small intestine, spleen, rib meat, sirloin and adipose tissues of a 12-month-old bull.
Comparative real-time PCR was performed using a Roter Gene 2000 system (Cobett Research, Australia) and a QuantiTect SYBR Green RT-PCR kit (QIAGEN, Germany) according to the manufacturers' instructions, with the following primer pairs: CRABP-I sense, 5'-AAGGCTTTGAGGAGGAGACC-3'; CRABP-I antisense, 5'-TCAGGATGAGTTCGTCGTTG-3'; CRABP-II sense, 5'-AAACAGGAGGGGGACACTTT-3'; CRABP-II antisense, 5'-CCCATTTCACCAGGCTCTTA-3'; 18S rRNA sense, 5'-CCCGAAGCGTTTACTTTGAA-3'; and 18S rRNA antisense, 5'-CCCTCTTAATCATGGCCTCA-3'.
Cloning and characterization of bovine CRABP-II cDNA
A cDNA encoding a bovine CRABP-II was isolated from bovine sirloin tissue by RT-PCR using degenerative primers.
The mRNA expression levels of CRABP-II were undetectable by Northern blot analysis using various bovine tissues (data not shown).
In contrast, bovine CRABP-II transcripts were detected in undifferentiated intramuscular fibroblast-like cells and their levels on day 15 had been induced approximately seven-fold compared to day 0 (Figure 4C).