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Molecular characterization of rabbit CPP32 and its function in vascular smooth muscle cell apoptosis.
Activation of the CED3/ICE-related protease CPP32 in cerebellar granule neurons undergoing apoptosis but not necrosis.
Immunohistochemistry was performed using primary antibodies against Caspase 3 (apopain, SCA-1, Yama, and CPP32 US Biological, USA) and anti-inducible nitric oxide synthase (anti-iNOS) (isoform Nitros Oxide) (dilution 1: 50; Santa Cruz Biotechnology) using streptavidin-biotin immunoperoxidase technique (DakoCytomation, California, USA).
Cohen, "Benzyloxycarbonyl-Val-Ala-Asp(OMe) fluoromethylketone (Z-VAD-FMK) inhibits apoptosis by blocking the processing of CPP32," Biochemical Journal, vol.
An anti-mouse monoclonal antibody Bcl-2 (C-2, sc-7382 (Santa Cruz Biotechnology Inc., USA) in dilution 1:200), an anti-mouse monoclonal antibody Bax (B-9, sc-7480 (Santa Cruz Biotechnology Inc., USA) in dilution 1:100), and an anti-mouse caspase 3 (CPP32) monoclonal antibody (clone JHM62 (Leica Biosystems Ltd., Great Britain) in dilution 1:50) were applied for 60 min at room temperature.
In human atherosclerotic carotid arteries, GDF-15 (immunoreactivity) was exclusively localized in activated macrophages and colocalized with oxLDL, MnSOD, AIF, caspase-3 (CPP32), PARP, c-Jun/AP-1, and p53.
Kamoshima et al., "Alteration of proteins regulating apoptosis, Bcl-2, Bcl-x, Bax, Bak, Bad, ICH1 and CPP32, in Alzheimer's disease," Brain Research, vol.
To study the apoptotic effect of Uncaria tomentosa and oxaliplatin, we analyzed the extrinsic pathway of apoptosis by evaluating the activities of caspase 8 (FLICE), caspase 1 (interleukin-1[beta] converting enzyme, ICE), and caspase 3 (CPP32).
Nakajima, "Induction of apoptosis and CPP32 expression by thyroid hormone in a myoblastic cell line derived from tadpole tail," Journal of Biological Chemistry, vol.
[H.sub.2][O.sub.2] may play a role as a mediator to induce apoptosis through release of cytochrome c to cytosol, activation of CPP32 protease, and PARP degradation (68).
Bax and Bcl-2 were purchased from Abcam, Germany, Caspase-3 (clone CPP32; NeoMarkers, USA).
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