Molecular characterization of rabbit CPP32
and its function in vascular smooth muscle cell apoptosis.
Activation of the CED3/ICE-related protease CPP32
in cerebellar granule neurons undergoing apoptosis but not necrosis.
Immunohistochemistry was performed using primary antibodies against Caspase 3 (apopain, SCA-1, Yama, and CPP32
US Biological, USA) and anti-inducible nitric oxide synthase (anti-iNOS) (isoform Nitros Oxide) (dilution 1: 50; Santa Cruz Biotechnology) using streptavidin-biotin immunoperoxidase technique (DakoCytomation, California, USA).
Cohen, "Benzyloxycarbonyl-Val-Ala-Asp(OMe) fluoromethylketone (Z-VAD-FMK) inhibits apoptosis by blocking the processing of CPP32
," Biochemical Journal, vol.
An anti-mouse monoclonal antibody Bcl-2 (C-2, sc-7382 (Santa Cruz Biotechnology Inc., USA) in dilution 1:200), an anti-mouse monoclonal antibody Bax (B-9, sc-7480 (Santa Cruz Biotechnology Inc., USA) in dilution 1:100), and an anti-mouse caspase 3 (CPP32
) monoclonal antibody (clone JHM62 (Leica Biosystems Ltd., Great Britain) in dilution 1:50) were applied for 60 min at room temperature.
In human atherosclerotic carotid arteries, GDF-15 (immunoreactivity) was exclusively localized in activated macrophages and colocalized with oxLDL, MnSOD, AIF, caspase-3 (CPP32
), PARP, c-Jun/AP-1, and p53.
Kamoshima et al., "Alteration of proteins regulating apoptosis, Bcl-2, Bcl-x, Bax, Bak, Bad, ICH1 and CPP32
, in Alzheimer's disease," Brain Research, vol.
To study the apoptotic effect of Uncaria tomentosa and oxaliplatin, we analyzed the extrinsic pathway of apoptosis by evaluating the activities of caspase 8 (FLICE), caspase 1 (interleukin-1[beta] converting enzyme, ICE), and caspase 3 (CPP32
Nakajima, "Induction of apoptosis and CPP32
expression by thyroid hormone in a myoblastic cell line derived from tadpole tail," Journal of Biological Chemistry, vol.
[H.sub.2][O.sub.2] may play a role as a mediator to induce apoptosis through release of cytochrome c to cytosol, activation of CPP32
protease, and PARP degradation (68).
Bax and Bcl-2 were purchased from Abcam, Germany, Caspase-3 (clone CPP32
; NeoMarkers, USA).