DNA extracts were diluted 1:10 with 10mM Tris-Cl, pH 8.5, and used as a template in PCR to amplify the 16S rRNA-encoding gene F2nR2 fragment with primers R16F2n/R16R2 and cpn60
Universal Target (cpn60
UT) sequence with primers H279p/H280p: D0317/D0318 (1:7 ratio) (Gundersen & Lee 1996; Dumonceaux et al.
A newly designed loop-mediated isothermal amplification (LAMP) assay based on cpn60
(encoding for chaperonin 60) was carried out in a total volume of 25 [micro]L containing 0.5 [micro]L each of pho-F3 and Pyo-B3 primer (25pmol/[micro]L) equivalent to 0.5 [micro]M final concentration, 1 [micro]L each of Pyo-LoopF and Pyo-LoopB primer (25pmol/[micro]L) equivalent to 1.0 [micro]M final concentration, 2 [micro]L each of Pyo-FIP and Pyo-BIP primer (25pmol/[micro]L) equivalent to 2.0 [micro]M final concentration, and 15 [micro]L Isothermal Master Mix Iso-001 (Optigene, Horsham, UK).
PCR reactions were performed for the seven housekeeping genes: gltA, gyrB, gdhB, recA, cpn60
, gpi, and rpoD as previously reported by Bartual et al.
tuberculosis is known to encode two molecular chaperones, Cpn60
.1 and Cpn60
.2, which function as modulators of myeloid cells among other regulatory functions .
Briefly, this method is based on analysis of 21 informative SNPs in 10 chromosomal loci (gltA, recA, cpn60
, gyrB, gdhB, rpoD, fusA, pyrG, rplB, and rpoB) and is used at the University of Oxford and the Pasteur Institute multilocus sequence typing (MLST) schemes.
) (groEL protein )/85 pyogenes (67) M3 8 Ornithine S.
Using primers to detect the mecA and nuc genes of Staphylococcus aureus and the cpn60
target on the chaperon gene of Kingella kingae, polymerase chain reaction (PCR) was performed using the purulent drainage obtained from the knee.
Bound to 30s ribosomal subunit 11 Binds to CPN60
in the presence of Mg- ATP and suppresses the ATPase activity of the latter 12 Binds to CPN60
in the presence of Mg- ATP and suppresses the ATPase activity of the latter 13 A molecular chaperone, thought to be involved in the initiation step of translation at high temperature.
Biochemical analysis, cpn60
and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34, isolated from pigs, are Streptococcus orisratti.
El tipo I de estas chaperoninas se encuentra en eubacterias, mitocondrias y cloroplastos, en donde se requiere generalmente la accion concertada de la chaperonina 60 (Cpn60
, GroEL) y la chaperonina 10 (Cpn10, GroES) (63, 65).
An organism with 95.5% sequence identity to Pseudomonas fluorescens was isolated on Mueller-Hinton agar with 2 [micro]g/mL meropenem and identified by partial sequencing of the cpn60
gene (GenBank accession no.