Effect of miR-142-3p on Cox-2
and PI3K/AKT/mTOR pathway in MLE-12 cells
Demonstration of COX-2
in tissue was done by indirect immunoperoxidase method.
We conducted a retrospective study of a cross-sectional historical cohort to evaluate the expression of VEGF and COX-2
markers in SCC of the larynx or hypopharynx and to correlate their expression with tumor size.
As we have indicated that our previous works demonstrated that COX-2
could be an important downstream of [beta]-catenin, we detected the expression of COX-2
in retinopathy caused by NMDA.
gene expression could be induced by a variety of growth factors and mitogens (Bakhle and Botting, 1996; Simon et al., 2000) which might lead it to be involved in carcinogenesis of many tumors, while there are few reports of COX-2
gene expression in feline cancers.
, ELISA, IL-6, Inflammation, Warfarin.
Collective evidence in human, animal, and cell culture studies clearly indicates that targeted inhibition of COX-2
is a viable approach for cancer prevention and treatment (PEEK, 2004).
Primers for COX-2
gene (10 exons, all the coding region) and primers for 5' (exon numbers 1, 2 and 12) and 3' region (exon numbers 18, 19, 20, 21 and 22) of BRCA1 gene were designed by primer 3 software.
Meanwhile, the immunohistochemical staining results of COX-2
, GLUT-1 and VEGF were recorded.
The COX-1 and COX-2
enzymatic inhibitory rates were performed by incubation of various compounds with human recombinant COX-1 and COX-2
(Cayman Chemical, Ann Arbor, MI).
The study's researchers, in a report published in Carcinogenesis, attributed this effect to curcumin--a natural COX-2
inhibitor--which is also turmeric's active constituent.
gene mediates inflammation, which in most cases helps our bodies eliminate pathogens and damaged cells.