He hard Ellyn Hibbens saye that that night she plucked of Cowps
(161) Mostyn v Fabrigas(1775) 1 Cowp 161, 173; 98 ER 1021, 1028.
This case (albeit referred to as Way v Yally) was cited in support of the proposition that the best way to establish a foreign law was to prove it as fact: Mostyn v Fabrigas (1775) 1 Cowp 161, 175; 98 ER 1021, 1029 (Mansfield C J).
All DHFR and COWP PCR products were sequenced, which confirmed the results of the RFLP diagnosis.
specimens) Mixed Participant COWP DHFR infection 0043D -- -- No 0214D -- -- No 0448D C.
parvum genotype in human fecal samples was first determined by a COWP gene-based PCR-RFLP tool (10).
Thirty-three of the 34 stool samples from outbreak A were amplified by both the COWP and SSU rRNA-based nested PCRs.
Genotyping was conducted by polymerase chain reaction (PCR) amplification of the internal transcribed spacer region, a hypervariable region in the 18S rRNA gene (4) and the COWP gene, (21).
PCR products were purified by using QIAquick spin columns (Qiagen, Mississauga, ON) according to the manufacturer's instructions before digestion with the restriction endonucleases Mse I (New England BioLabs, Mississauga, ON) for the ITS 1 locus and Rsa I (New England BioLabs) for the COWP gene.
Polymorphic genes that identify these genotypes include the Cryptosporidium oocyst wall protein (COWP) gene (5), the thrombospondin-related adhesive proteins Cryptosporidium-1 (TRAP-C1 ), and Cryptosporidium-2 (TRAP-C2 ).
We have described the identification by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of a single human isolate with an unusual COWP genotype, designated genotype 3 (10).
PCR-RFL the Cryptosporidium oocyst wall protein (COWP
) gene discriminates [ILLEGIBLE TEXT] and C.