Sequence-specific primers and fluorescence-labeled probes complementary to EI24, YWHAE, TFRC, CORO1C, S100A16, NLK, RAB1B, DEDD, CUL5, and PRDX3 and the endogenous reference GAPDH were used.
These were tyrosine 3-Monooxygenase (YWHAE, aka 14-3-3 protein epsilon), transferrin receptor (TRFC), coronin, actin binding protein 1C (CORO1C), and periredoxin 3 (PRDX3).
YWHAE, TRFC, CORO1C, and PRDX3 were predicted to be elevated while, NLK, RAB1B, DEDD, CUL5, EI24, and S100A16 were predicted to be reduced in PCa cells as compared to PNF-08 cells.