Consistent with this, it also enhanced transcript levels of M2 macrophages markers such as Mrc1 (Figure 3(c)) and Clec10a (Figure 3(d)), while it reduced transcript levels of the M1 macrophage marker CD274 (Figure 3(e)).
As shown in Figure 5, hemin treatment increased levels of HO-1 transcripts (Figure 5(a)) and protein (Figure 5(c)) in the adipose tissue and also transcripts of M2 marker genes (Mrc1 and Clec10a) (Figure 5(b)) while it decreased M1 marker expression (CD274 and NOS2) (Figure 5(b)).
Secondly, adiponectin overexpression either had no effect on (Figures 7 and 8) or significantly reduced (Figure 3) OVA-induced increases in the expression of Retnla, Chi3l3, and Clec10a
. In contrast, in macrophages from other sources, including peritoneal macrophages, adipose tissue macrophages, Kupffer cells, bone marrow or monocyte-derived macrophages, and macrophage cell lines, adiponectin augments the induction of these M2 genes either on its own or in conjunction with Th2 cytokines [27-29].