(2c) EpCAM, CD24, CA19- 9, CLDN3
, CA-125, MUC18, EGFR, and HER2, a list of putative ovarian cancer markers; CD41 and CD45 for immune host cell markers and D2-40 a mesothelial marker for exosomes profiled left in nPLEX sensor and their parental ovarian cell lines filled in right for flow cytometer, according to Im et al.
Fu et al., "CLDN3
inhibits cancer aggressiveness via Wnt-EMT signaling and is a potential prognostic biomarker for hepatocellular carcinoma," Oncotarget, vol.
Little to no expression was observed at any time point in either the biopaper or PET coculture systems for genes gap junction [beta]4 (GJB4), gap junction [beta]6 (GJB6), tight junction protein 3 (TJP3), and claudin 3 (CLDN3) (Table 2).
Expression of only endothelial, not epithelial, tight junction genes was confirmed though the use of negative controls TJP3 and CLDN3. TJP3, which codes for the tight junction protein zonal occludens-3 (ZO-3), is primarily expressed at epithelial tight junctions .
Many genes related to cell junctions (Tnfa, Tgf[beta], Actin, Tubulin, E-adherin, Tjpl, Tjp2, Tnfr, Integrin, Epsin, Pvrl, Rho Gtpase, Cadherin, Cldn3
, Ras and A2m) diversely altered depending on pesticide doses and exposure times, are listed in Table S2 (http://dx.doi.
Gene (a) Symbol Description NKX2-1 (TTF-1) NK2 homeobox 1 (thyroid transcription factor 1) SFTA3 Surfactant associated 3 SFTA2 Surfactant associated 2 LMO3 LIM domain only 3 (rhombotin-like 2) XAGE1D X antigen family, member 1D CLDN3
Claudin 3 Median expression value (b) Symbol Adenocarcinoma Squamous cell carcinoma NKX2-1 (TTF-1) 2956.61025 82.2006 SFTA3 2412.19165 66.691 SFTA2 1951.85825 41.01335 LMO3 1901.15055 95.90595 XAGE1D 1873.0292 5.0326 CLDN3
1647.12575 93.83215 (a) All the genes conforming to the following two conditions are listed: (1) median expression value of the gene >1500 in adenocarcinoma and (2) median expression value of the gene <100 in squamous cell carcinoma.
(47,148-150) Other molecules recently proposed as possible targets for novel therapies of PENs are CDK4, PDGFR-[beta], CLDN3
, and CXCL-12.