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Histology revealed a moderately differentiated adenocarcinoma of rectum, CK7 was negative.
Immunostaining results showed calretinin (−), CK7 (+), and paired-box gene 8 (PAX-8) (+) [Figure 1]b,[Figure 1]c,[Figure 1]d.
The tumor cells react with CK7, CK5/6, and EM A, and will also show strong TTF1 immunoreactivity (figure 2).
2] and CK20 have been shown to be positive in up to 21%/21% of gastric and 14%/21% of ovarian mucinous adenocarcinomas, respectively, and CK7 was not particularly helpful in this differential, as it was positive in only about 50% of gastric and ovarian mucinous adenocarcinomas.
Immunohistochemistry staining of the tumor for CK7, CK20, and CDX2 can also be employed to help make this determination.
The tumour cells were positive for CK7 and CK20 (figure 2A and B) and negative for CDX2, PSA and AMACR.
The tumour was strongly positive for CK7 and CK19 but focally positive for CK20.
The neoplastic cells were positive for pan-cytokeratin (CK) and CK7 and showed focally immunoreactivity for beta-hCG [Figure 5] and human epidermal growth factor receptor 2 (Her-2) [Figure 6].
Immunohistochemical examination of the specimen was positive for cytokeratin (CK)18, CK19, CK5/6 and CD5 and CD117, and negative for CK7, TTF1, CD56 and CD20 markers (Figure 4).
2B), c-kit, Vim, CEA, CgA, S-100, CD117 and alpha-fetoprotein (weakly positive), but negative for EMA, Syn, CD20, CD30, placental alkaline phosphatase, CK7, CK2, and a-inhibin.
They were positive for CK7 and negative for CK20, strongly positive for oestrogen receptors and HER2 negative.
Adjacent and admixed to these areas there were also foci of lung adenocarcinoma with a predominant acinar growth pattern (Figure 5) expressing TTF-1 and CK7 (Figures 6(a) and 6(b)).
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