3 Immunohistochemically, epithelium shows positive expressions of cytokeratins (CK), especially CK5
, CK14, and CK19, and mesenchymal tumor cells show positive expression of nestin.
Epithelial components were stained by immunohistochemistry for cytokeratin 7, EMA, and vimentin, and less so for CK5
, GFAP, NSE, and S-100 (Figure 2).
Also reported was a large inverted component, the presence of small nests of urothelial cells in the stalk, and the strong staining for CK5
Luminal cells in both humans and mice are characterized by the expression of low molecular weight cytokeratin (CK) 8 and 18, and androgen receptor (AR), while basal cells express the high molecular weight CK5
and p63 in both species [3,34,36-38].
On immunohistochemistry the tumour was positive for calretinin (Figure 3), CK5
(Figure 4), CK7 (Figure 5) and negative for CK 20.
is More Sensitive than CK5
/6 in Identifying "Basal-like" Phenotype of Breast Carcinoma.
When gene expression data are compiled from different sources, basal-like tumors in TNBC consistently cluster within a group that express CK5
, 6, 14, 17, and caveolin1/2, as well as c-kit and P-cadherin (15,42).
3] % % % 1 2 3 4 5 CK0 1727 14,2 34,8 24,5 CK1 1684 18,5 36,5 31,1 CK2 1701 17,7 35,8 30,2 CK3 1672 18,5 36,9 30,9 CK4 1673 18,6 36,9 31,2 CK5
1672 18,7 36,9 31,3 CK6 1673 18,3 36,9 30,6 Poringumo Sudeties [P.
These, in combination with some of the more traditional markers such as TTF-1, CK5
and p63, may enable much more confident identification of SqCC and LADC.
The chapter on mammospheres and breast carcinomas describes the basal like phenotype of breast CSC with expression of CD44, CK5
35,36) Non expression of CK5
may be an early event occurring in tobacco-associated pathological changes in the buccal mucosa.
These cadmium-transformed breast epithelial (CTBE) cells displayed characteristics of basal-like breast carcinoma, including ER-(X negativity and HER2 (human epidermal growth factor receptor 2) negativity, reduced expression of BRCA1 (breast cancer susceptibility gene 1), and increased CK5
(cytokeratin 5) and p63 expression.