According to the company, DSIa[euro](tm)s workforce will use the CK3
for client data collection and inventory management, including in-store magazine implementation and ongoing merchandising efforts in over 7,000 weekly locations across the USA.
The primary antibodies used included anti-human p63[alpha] antibody (Cell Signaling, Beverly, MA), anti-human ABCG2 antibody (Abcam, Cambridge, England), anti-human ABCB5 antibody (Invitrogen, Carlsbad, CA), anti-human CK19 antibody (Abcam, Cambridge, England), anti-human CK3 antibody (Abcam, Cambridge, England), and anti-human desmoglein 3 antibody (Novus Biologicals, Littleton, Co.).
The primary antibodies included anti-human p63[alpha] (Cell Signaling), anti-human ABCG2 (Abcam), anti-human ABCB5 (Invitrogen), anti-human CK3 (Abcam), and anti-human desmoglein 3 (Novus Biologicals).
In contrast, expression of the corneal epithelial cell markers CK3 and CK12 increased when [ABCG2.sup.+]/ [ABCB5.sup.+] LESCs were cultured in CnT20 and CnT30 (Figure 2(c)).
When cultured in 10% serum DMEM, [ABCG2.sup.+]/[ABCB5.sup.+] LESCs expressed the stem cell-specific markers ABCG2 and P63[alpha], but when cultured in CnT30, P63[alpha] expression decreased and expression of the corneal epithelial cell marker CK3 increased (Figure 3(a)).
In summary, both of RT-PCR and immunocytochemistry indicated that COMECs could express molecular markers (CK3, p63, ABCG2) identical on APCS and HAM.
Our studies also indicate that both of the sheets express markers of stratified epithelia CK3 and the progenitor cell marker p63 and the putative stem cell marker ABCG2.
It shows the expression of p63 in the nuclei of the basal epithelial sheet and ABCG2 and CK3 expression in the cytoplasm in the fullthickness in cultivated oral mucosal epithelial cell sheets both on APCS or HAM.
Name Primer CK3 Forward Reverse ANP63a Forward Reverse ABCG2 Forward Reverse Name Sequence CK3 5-ACCTGGGAAAGCACGAGAA-3' 5-GGTCAGCGTTGGAGACATCA-3' ANP63a 5-GAGGTTGGGCTGTTCATCAT-3' 5-AGGAGATGAGAGGGGAGGA-3' ABCG2 5'-TGGTGCCCTTTACTTTGGTC-3' 5-ACACTTGGCAAGAACCTCAT-3' Name Product size (bp) CK3 120 ANP63a 188 ABCG2 234 Figure 3: Quantitative real-time PCR analysis of comparison of putative epithelial cell marker expression.