The basal cells adjacent to the basement membrane are the least differentiated and express the basal cytokeratin (CK) 5/6 and CK14 but not CK18 and CK20
, which are characteristic of more differentiated intermediate urothelial cell layers.
Pancytokeratin, LCA, CK7, CK20
, and uroplakin were negative.
In our study of 60 cases, 24 (40%) cases of squamous cell carcinoma being the most common with P63 positivity, 20 (33%) cases of adenocarcinoma with TTF-1, CK 7, EGFR and Napsin A positivity with negative P63 and 15 (25%) cases of small cell lung carcinoma with TTF-1 positivity and Napsin A negativity and 1 (1.66%) case of metastasis from colon adenocarcinoma with CK7 negativity and CK20
positivity were confirmed and out of 60 cases, 39 (65%) were males and 21 (35%) were females.
The immunohistochemistry staining by CK7, CAM5.2, 34 beta E12, CEA, C-A125, Leu-M1 and Vimentin (positivity), P53 and Bcl-2 (overexpression) and CK20
, [beta]-HCG and Alpha 1-fetoprotein (negative staining) are suggestive for CCC and could distinguish this malignancy from other differential diagnoses such as Aria-Stella reaction, micro glandular hyperplasia, and mesonephric hyperplasia in adults, and Yolk sac tumor, sarcoma botryoides, and embryonal rhabdomyosarcoma in children, and metastatic renal cell carcinoma in all ages.
Immunohistochemical staining was positive for p40 and elevated ki-67 but negative for GATA3, p16 and CK20
. The differential diagnosis was for condyloma acuminatum versus urothelial hyperplasia and the pathology was sent to Stanford University for consultation read.
Immunohistochemistry indicated negative reaction to CK7, CK20
and PAN-CK, and positive reaction to S-100, HMB-45, tyrosinase and MITF.
Immunohistochemical analysis revealed a panel indicative of intestinal epithelial histogenesis with immunoreactivity to CK20
and CDX2 markers.
Immunohistochemistry positive for CK20
, CER and negative for GCDFP, ER, PR, CK7 is a strong predictor of primary gastric adenocarcinoma instead of primary breast tumor1.
The PMA shows positive immunostaining with CK7 and a negative immunostaining for CK20
, that is akin to the immunostaining pattern of the breast carcinoma, whereas metastatic adenocarcinoma from the gastrointestinal tract is CK7 negative and CK20
The tumor cells were immunoreactive for CK7, CK19, CEA, and EMA but were nonimmunoreactive for CK20
Gonderilen biyopsi ornegi kesitlerde H&E (Resim 1, 2) ile boyali kesitlerde fibromuskuler dokuyu infiltre eden uniform gorunumde kucuk hiperkromatik nukleuslu eozinofilik sitoplazmali bir kismi plazmasitoid gorunumde atipik hucreler dikkati cektigi ve bunun uzerine yapilan immunhistokimyasal incelemelerde bu hucrelerde PanCK, 34BE12 (Resim 3), CK7 ve CD138 (Resim 4, 5) ile yaygin p63 (Resim 6) ile fokal ekspresyon izlenirken Kappa, Lambda, CK20
, Trombomodilin, LCA, Kromogranin A, CD56 ve CEA ile ekspresyon gorulmedigi ve sonuc olarak mevcut histomorfolojik ve immunhistokimyasal bulgularin olguda malign bir epitelyal tumor ve oncelikle plazmasitoid varyant urotelyal karsinomu dusundurdugu raporlandi.
Immunohistochemically neoplastic cells displayed CK20
, CD138, CD56, and chromogranin positivity; weak pancytokeratin positivity was noted.