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Immunohistochemical staining with CK7 and CK20 were positive.
In UCIS, CK20 will generally show full-thickness staining of the urothelium, whereas benign/reactive urothelium shows CK20 positivity in the umbrella cell layer only.
HER-2/neu can be added to a panel of CK20 and p53 to help to differentiate reactive atypia from carcinoma in situ in difficult cases.
Immunohistochemistry staining of the tumor for CK7, CK20, and CDX2 can also be employed to help make this determination.
The tumour cells were positive for CK7 and CK20 (figure 2A and B) and negative for CDX2, PSA and AMACR.
Quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood of colorectal cancer patients.
The tumour was strongly positive for CK7 and CK19 but focally positive for CK20.
Immunohistochemical studies were carried out on the cell block and core biopsy sections using antibodies TTF-1 (1:200; Leica), thyroglobulin (1:1000; Dako), ER (1:50; Dako), PR (premade; Leica), GATA3 (1:200; Cell Marque), GCDFP-15 (1:500; Leica), CD56 (Premade; Leica), synaptophysin (1:200; Dako), chromogranin (1:200; Cell Marque), pAx8 (1:100; Cell Marque), villin (1:100; Leica), CK 7 (1:400; Dako) and CK20 (1:200; Novocastra).
The specimen was positive for CD99, CD56, and synaptopysin, and negative for CK7, CK20, and AE1/AE3--confirming EWSR gene rearrangement and an ES.
They were positive for CK7 and negative for CK20, strongly positive for oestrogen receptors and HER2 negative.
20) Positivity for Hep Par-1 and negativity for CK7/ CK20 strongly favored HCC over metastasis, and negativity for Hep Par-1 and positivity for CK7/ CK20 confirmed the diagnosis of metastasis.
2), CK7, CK20, CDX-2, chromogranin, synaptophysin, TTF-1, desmin, smooth muscle actin, CD34, and HMB-45.
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