The basal cells adjacent to the basement membrane are the least differentiated and express the basal cytokeratin (CK) 5/6 and CK14
but not CK18 and CK20, which are characteristic of more differentiated intermediate urothelial cell layers.
The grafts were assessed for the presence of progenitor cells, and the predominant phenotype (>50%) consisted of small cells positive for [DELTA]Np63, CK14
, and ABCG2 and negative for CK3/12 and desmoglein 3.
SCMC is often positive for basal cytokeratins and hence the importance of including basal markers such as p63, CK5, and CK14
in the panel if the diagnosis of metaplastic carcinoma is considered [5, 14].
3 Immunohistochemically, epithelium shows positive expressions of cytokeratins (CK), especially CK5, CK14
, and CK19, and mesenchymal tumor cells show positive expression of nestin.
In the well-differentiated case of SCC, CK1 and CK10 expressions were downregulated, whereas CK14
expression was upregulated.
Expression of CK5/6, CK14
and CK20 correlate with high tumor grade.
Immunohistochemical analyses included staining with Ki-67, p53, epidermal growth factor receptor (EGFR), cytokeratin (CK) 8, CK14
, CK17, and CK18.
Immunhistochemical testing can be helpful to differentiate between thymoma and thymic lymphoma by labeling cytokeratins CK14
and CK18, as well as showing positive results for proliferating cell nuclear antigen in cases of thymomas.
On histological and immunohistochemical examination, the lesion was negative CTK, CK5/6, CK7, CKLMW8/18, p63, and CK14
. The lesion was diagnosed as a primary osteosarcoma of the breast.
Para el analisis de los casos, se agruparon los 22 marcadores utilizados en cuatro grupos: a) marcadores de la clasificacion molecular (ER, PR y HER2), b) marcadores de proliferacion celular y otros (EGFR, C-Kit, p63, survivina, ALDH1, CD10 y Ki-67), c) marcadores de filamentos intermedios (Ck5/6, CK8/18, CK14
, CK17, CK APM y vimentina), d) marcadores de adhesion celular y citoesqueleto (cadherina E, cadherina P, p120, actina de musculo liso, alfa actina y calponina).