KRT18

(redirected from CK-18)

KRT18

A gene on chromosome 17q21.2 that encodes a type-I keratin, an intermediate filament protein. Together with its filament partner keratin 8, they are the most commonly expressed of all intermediate filaments, and found in tissues lined by single layer epithelia.
 
Molecular pathology
KRT18 mutations are associated with cryptogenic cirrhosis.
References in periodicals archive ?
The sixth paper identified a novel biomarker cytokeratin 18 epitope M30 (M30 CK-18), which was correlated with liver inflammatory activity.
Serum caspase-cleaved fragmented cytokeratin-18(CK-18) reflects the degree of apoptosis and has been shown as an independent predictor in diagnosis of NASH [15-18].
Among the RNA cargoes identified by a miRNA microarray analysis, we found that miR-503-3P inhibited stemness via downregulation of Nanog, a pluripotent marker, and upregulation of CK-18, a differentiation marker, resulting in significantly reduced tumor growth in vivo.
Serum CK-18 M30 concentration was detected by enzyme-linked immunosorbent assay (ELISA, PEVIVA, Bromma, Sweden) according to the manufacturer's instructions.
Immunohistochemical staining was used to assess collagen deposition ([alpha]-SMA, MMP-9), mesothelial cell healing (CK-18), and gastrointestinal dynamics (C-kit) and was performed using a streptavidin-biotin kit (Maxim, Fuzhou, China) following the manufacturer's instructions.
This system is based on a combination of immunocytochemistry and immunofluorescence techniques, in which the immunomagnetic selection of CTCs with antibodies specific for the epithelial marker EpCAM is followed by staining of EpCAM-positive cells for CKs (CK-8, CK-18, and CK-19).
In a prospective study of 59 randomly selected patients with chronic hepatitis C virus (HCV) infection, a high degree of inflammatory liver injury on liver biopsy was associated with greater levels of the products that result from the cleavage of cytokeratin-18 (CK-18, a major intermediate filament protein in liver cells) by caspases during apoptosis (Hepatology 2004;40:1078-87).
El efecto agudo de Malathion en inyeccion intraperitoneal unica, de 1/12 la LD50 en raton fue analizado 1,8,16,35 y 40 dias despues de la inyeccion, evaluando proliferacion y apoptosis de las celulas espermatogenicas y dano de las celulas de Sertoli, estudiados por la deteccion inmunocitoquimica de citoqueratina (usando CK-18).
Recently, one important review [8] summarized several novel circulating biomarkers including cytokeratin 18 (CK-18), fibroblast growth factor 21 (FGF-21), and related biomarker panel combining more than two kinds of serum proteins.
After antigen retrieval chamber Pascal (Dako Corporation, Carpinteria, CA, USA) and blocking of endogenous peroxidase activity, the sections were incubated in primary antibody anti-a smooth muscle actin ([alpha]-SMA) (1:50, Dako), anticytokeratin-18 (CK-18) (1:100 Bioss), and antivimentin (VIM) (1:100, Bioss).
Cells were then incubated in blocking solution overnight at 4[degrees]C with primary antibodies as follows: rabbit polyclonal anti-human albumin (ALB) and mouse monoclonal anti-human cytokeratin 18 (CK-18) (all 1:100) (DakoCytomation, Glostrup, Denmark).