The sixth paper identified a novel biomarker cytokeratin 18 epitope M30 (M30
CK-18), which was correlated with liver inflammatory activity.
Serum caspase-cleaved fragmented cytokeratin-18(
CK-18) reflects the degree of apoptosis and has been shown as an independent predictor in diagnosis of NASH [15-18].
Among the RNA cargoes identified by a miRNA microarray analysis, we found that miR-503-3P inhibited stemness via downregulation of Nanog, a pluripotent marker, and upregulation of
CK-18, a differentiation marker, resulting in significantly reduced tumor growth in vivo.
Serum
CK-18 M30 concentration was detected by enzyme-linked immunosorbent assay (ELISA, PEVIVA, Bromma, Sweden) according to the manufacturer's instructions.
Immunohistochemical staining was used to assess collagen deposition ([alpha]-SMA, MMP-9), mesothelial cell healing (
CK-18), and gastrointestinal dynamics (C-kit) and was performed using a streptavidin-biotin kit (Maxim, Fuzhou, China) following the manufacturer's instructions.
This system is based on a combination of immunocytochemistry and immunofluorescence techniques, in which the immunomagnetic selection of CTCs with antibodies specific for the epithelial marker EpCAM is followed by staining of EpCAM-positive cells for CKs (CK-8,
CK-18, and CK-19).
In a prospective study of 59 randomly selected patients with chronic hepatitis C virus (HCV) infection, a high degree of inflammatory liver injury on liver biopsy was associated with greater levels of the products that result from the cleavage of cytokeratin-18 (
CK-18, a major intermediate filament protein in liver cells) by caspases during apoptosis (Hepatology 2004;40:1078-87).
El efecto agudo de Malathion en inyeccion intraperitoneal unica, de 1/12 la LD50 en raton fue analizado 1,8,16,35 y 40 dias despues de la inyeccion, evaluando proliferacion y apoptosis de las celulas espermatogenicas y dano de las celulas de Sertoli, estudiados por la deteccion inmunocitoquimica de citoqueratina (usando
CK-18).
Recently, one important review [8] summarized several novel circulating biomarkers including cytokeratin 18 (
CK-18), fibroblast growth factor 21 (FGF-21), and related biomarker panel combining more than two kinds of serum proteins.
Caspases cleave cellular proteins, including cytokeratin-18 (
CK-18).
After antigen retrieval chamber Pascal (Dako Corporation, Carpinteria, CA, USA) and blocking of endogenous peroxidase activity, the sections were incubated in primary antibody anti-a smooth muscle actin ([alpha]-SMA) (1:50, Dako), anticytokeratin-18 (
CK-18) (1:100 Bioss), and antivimentin (VIM) (1:100, Bioss).
Cells were then incubated in blocking solution overnight at 4[degrees]C with primary antibodies as follows: rabbit polyclonal anti-human albumin (ALB) and mouse monoclonal anti-human cytokeratin 18 (
CK-18) (all 1:100) (DakoCytomation, Glostrup, Denmark).
