In parotid glands, ethanol consumption affected expression of CK-18 at one week and four weeks of exposure (Figure 4(a)).
On the other hand, submandibular gland showed no difference between the control and ethanol groups in both periods of analysis regarding the expression of CK-18 (Figure 4(b)).
In line with a heavy chronic model of exposure to EtOH, morphologic changes after binge drinking are more related to expression patterns of CK-18 and [alpha]-SMA.
Parotid glands showed a reduction in CK-18 expression in 1- and 4-week episodic binges, while submandibular glands did not present any significant modification.
This system is based on a combination of immunocytochemistry and immunofluorescence techniques, in which the immunomagnetic selection of CTCs with antibodies specific for the epithelial marker EpCAM is followed by staining of EpCAM-positive cells for CKs (CK-8, CK-18
, and CK-19).
Los resultados muestran disminucion del numero de celulas germinales, seguida de una proliferacion compensatoria de espermatogonias al dia 16 (como lo muestra la incorporacion de timidina H3 al testiculo), aumento de la tasa de apoptosis (especialmente de espermatogonias y espermatocitos en preleptoteno), la reactividad a CK-18 de las celulas de Sertoli que denota alteracion de ellas, probablemente asociada a dano de la linea germinal que se observa en los animales tratados a intervalos cortos.
The acute effects of malathion (single intraperitoneal injection), 1/12 the LD50 to mice were analyzed at l, 8, 16, 35 and 40 days after injection, in terms of spermatogenic cell proliferation and apoptosis and of Sertoli cell compromise as revealed by immunocytochemical detection of CK-18 (cytokeratin).
Sertoli cell reactivity to CK-18, denoting alteration of them, probably due to germ cell damage, was seen in treated mice.
Testicular sections of control and experimental mice were treated by the method of TUNEL, to evaluate apoptosis, according to Sasso-Cerri & Miraglia (2002) and with immunohistochemical reaction for cytokeratin CK-18, as stated by Steger et al.
CK-18 cytokeratin was positive in peritubular and Leydig cells of control mice (Fig.