DDIT3

(redirected from CHOP10)

DDIT3

A gene on chromosome 12q13.1-q13.2 that encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors, which form heterodimers with other C/EBP members—e.g., C/EBP and LAP—preventing their DNA binding activity. DDIT3 is activated by endoplasmic reticulum stress, and plays roles in adipogenesis, erythropoiesis and apoptosis.
Mentioned in ?
References in periodicals archive ?
Rg1 reduces lipid accumulation and ROS production via the activation of C/EBP-homologous protein 10 (CHOP10), which attenuates fat accumulation and downregulates protein levels of NADPH oxidase 4 (NOX4).
Furthermore, several negative regulators, including C/EBP homologous protein 10 (CHOP10) and GATA-binding proteins 2 and 3 (GATA2/3) have been shown to attenuate adipogenesis (Batchvarova et al.
In addition, some negative regulators such as CHOP10, GATA 2/3, and TNF-[alpha] play an important role in the regulation of adipogenesis (Batchvarova et al.
Collectively, our data showed that CT can effectively inhibit adipogenesis via inhibition of key adipogenic transcription factors (such as C/EBP[beta], PPAR[gamma], C/EBP[alpha], adiponectin, and aP2) and via induction of several negative regulators such as GATA2, CHOP10, and TNF-[alpha].
Association between arsenic suppression of adipogenesis and induction of CHOP10 via the endoplasmic reticulum stress response.
Although the expression of C/EBP [beta] rises quickly in preadipocytes in response to adipogenic hormones, its DNA-binding activity is initially suppressed through binding with C/EBP homologous protein (CHOP10, also known as C/EBP[zeta], DDIT-3, or GADD153) (Rosen and MacDougald 2006).
Antibodies for C/EBP[beta] (sc-61; 1:500), C/EBP[beta] (sc-7962; 1:500), CHOP10 (sc-575; 1:500), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, sc-20357; 1:500) were obtained from Santa Cruz Inc.
Upon exposure to adipogenic signals, such as DMI or DMIRI cocktail, C/EBP[beta] and C/EBP[delta] are rapidly expressed and transcriptionally regulate the expression of PPAR[gamma] and C/EBP[alpha], whereas C/EBP[euro] and CHOP10 serve as negative regulators of PPAR[gamma] transcription (Batchvarova et al.
To investigate whether UPR, especially CHOP10 induction, is involved in the suppression of terminal adipogenesis by [iAs.sup.3+], the expression of ATF4 and CHOP10 were measured in 3T3-L1 cells.
CHOP10 induction in adipogenic suppression by arsenic.
Although the expression of C/EBP[alpha] rises quickly in preadipocytes in response to adipogenic hormones, its DNA-binding activity is initially suppressed through binding with CHOP10 (Tang and Lane 2000).
Among the genes that are transcriptionally regulated by ATF4, ATF6, and XBP1, CHOP10 is one of the mostly expressed inducible genes during ER stress (Oyadomari and Mori 2004).