In contrast, the effect of betamethasone dipropionate on the epidermis was restricted to a normalization of differentiation with a highly significant increase of keratin-10 positive epidermal surface without a significant effect on Ki- 67 positive nuclei, and the effect on T- cell subsets was restricted to a reduction of natural killer T-cell receptors designated by CD94
and CD161 in the epidermis.
Target antigen Fluorophore Clone Company, address CD28 FITC CD28.2 CD56 FITC NCAM16.2 CD69 FITC FN50 CD94
FITC HP-3D9 CD95 FITC DX2 PD-1 FITC MIH4 PD-1 BV421 MIH4 TCR[alpha][beta] FITC T10B9.1A-31 CD8 FITC SK1 CD8 APC Cy7 SK1 BD Bioscience CD8 V500 RPA-T8 Franklin Lakes, NJ, USA CD45RO APC UCHL1 CD4 Alexa Fluor 700 RPA-T4 CD3 PE UCHT1 CD3 V450 UCHT1 CD3 BV510 UCHT1 CD19 PE HIB19 CD27 PE M-T271 CD27 BV421 M-T271 CCR7 PE-Cy7 3D12 TCR[gamma][delta] FITC IMMU510 Beckman Coulter Inc.
Regarding the expression of activating and inhibitory receptors, it was shown that the expression of CD57, a marker of terminally differentiated NK cells, NKG2D, and CD94
, was not altered on NK cells from aMCI and mAD patients compared to elderly healthy donors.
The expression of other peripheral blood NK cell surface receptors including CD16, CD94
, NKG2A, 2B4, CD158a/h, CD158b, and CD158e1 was not significantly altered between GC patients and healthy donors (Figure 1 and Table 1).
Changes in endometrial natural killer cell expression of CD94
, CD158a and CD158b are associated with infertility.
Increase of CD69, CD161 and CD94
on NK cells in women with recurrent spontaneous abortion and in vitro fertilization failure.
NKG2E forms heterodimeric complexes with CD94
. These complexes interact with HLA-E, a major histocompatibility complex (MHC) class Ib protein, which is involved in pathogenesis of T1DM.
Flow cytometry (FCM) analysis of the BM aspirate cells using the EuroFlow lymphoid screening tube (LST) and antibody panel for NK cell chronic lymphoproliferative diseases (NK-CLPD) , complemented with other cell surface markers, showed that the neoplastic cells were positive for CD45 (high), CD2, CD26, CD38, CD94
(high), and HLADR (high, heterogeneous) antigens, and negative for surface CD3, TCR, CD4, CD5, CD7, CD8, CD11b, CD11c, CD16, CD56, CD57, and CD161, as well as for cytoplasmic CD3; in addition, they express intracellular granzyme B and perforin (Figure 3).
Hepatosplenic T-cell lymphoma shows phenotypic aberrancy through frequent loss of CD5 and/or CD7, as well as reactivity with multiple killer immunoglobulin-like receptors and a weak to negative reaction to CD94
. (13,15) The neoplastic cells express molecules associated with cytotoxic T cells, such as TIA1.
CD69 is a stimulatory receptor for natural killer cell and its cytotoxic effect is blocked by CD94
1A transcripts characterize lymphoblastic lymphoma/ leukemia of immature natural killer cell origin with distinct clinical features.