Binding of the FITC-labelled antibodies against CD4 and CD8 is not reduced by increasing concentrations of AGP, indicating lacking competition of AGP with the antibodies for the same epitopes on CD4 and CD8 antigen
. Furthermore it is most probable that AGP does not interact with any other epitopes on CD4 and CD8 antigen
, because an AGP-CD4 or -CD8 receptor complex would inhibit binding of CD4- or CD8-antibodies due to size of AGP with a molecular weight of 1.2 x [10.sup.6] Da.
Our patient did not show expression of CD25 on is B cells, in contrast to most of the published cases.[10,12] In one report of B-CLL, CD8 expression on B cells was suggested as a marker of aggressive disease; however, other reports did not show any difference in presentation or survival of these patients when compared with patients with classic B-CLL, strongly suggesting that CD8 antigen is not a marker of aggressive disease.
Aberrant CD8 antigen expression in a patient with chronic lymphocytic leukaemia showing unusual disease progression.
B-cell chronic lymphocytic leukemia with CD8 expression: report of 10 cases and immunochemical analysis of the CD8 antigen. Br J Haematol.