These genes include SERPINE2 , CD79A
, and POU2AF1.
The monoclonal antibodies (MoAb) used for diagnosis of AL were: for B-lineage: CD19, CD22, CD20, CD10, for T-lineage: CD2, CD3, CD5, CD7, CD4, CD8, for myelo-monocytic lineage: CD13, CD33, CD11c, CD64, CD14, other cells surface markers: CD34, CD117, HLA-DR and intracellular MoAb: CD79a
, CD3 and MPO.
Acute myeloid leukemia with t(8;21) often expresses the B-cell markers CD19, CD79a
, and PAX5, which is associated with transcriptional upregulation of the PAX5 gene.
These cells expressed CD79a
(Figure 1H) and BOB-1 on immunohistochemical staining, but did not express CD20.
The permeabilizing solution containing 15% formaldehyde and 50% diethylene glycol and proprietary permeabilizing agent used for intracellular staining of antigens such as MPO, CD79a
, CD3 Cytoplasmic and Tdt.
La expresion positiva de CD79a
y MUM1 y negativa de PAX5, CD3 y lisozima concluyeron el diagnostico de linfoma B inmunoblastico.
The large cells with atypical lymphoid characteristics displayed a B-cell phenotype reaction with vimentin, LCA (CD45), CD20 (Figure 1C), and CD79a
This diagnosis was based on morphology and molecular marker positive for CD20, CD79a
, Ki67, Bcl2, and negative or normal patterns for cyclinD1, CD34, CD3, and CD30.
Results of immunohistochemical staining for CD3 and CD79a
showed the neoplastic cells were CD3 (Fig 3B) and CD79a
-, findings that are consistent with a T-cell lymphoma.
4) and positive Bc-16 as well as positive CD79a
and CD10 in cases of Burkitt lymphoma.
All other tested markers (TCA, CD3, CD79a
, chromogranin A, synaptophysin, myogenin, desmin, S-100 protein, TdT, LMP1, CD99, TTF-1 and CK-14) were negative, except for a weak expression of neuron-specific enolase (NSE).
To help distinguish seminoma from malignant lymphoma, we added immunostains for CD79a
(result: negative) and SALL4 (positive; Figure 4).