CD166 and CD71
are among cancer targets that are considered to be inaccessible to conventional antibody drug conjugates due to their presence on many healthy tissues.
Pursuant to the April 2016 Co-Development and Licensing Agreement, CytomX and AbbVie are also co-developing CX-2029, a PDC against CD71
, also known as transferrin receptor 1, is a cell surface protein that is essential for iron uptake in dividing cells, is highly expressed in a number of solid and hematologic cancers and has attractive molecular properties for efficient delivery of cytotoxic payloads to tumor cells.
Eight-color MFC was performed in all patients as a routine clinical test on BM samples that were obtained as part of baseline assessment at the time of diagnosis, the end of the first and second courses of chemotherapy, as well as pre-HSCT., A panel of eight antibody combinations that recognize CD7, CD11b, CD13, CD14, CD16, CD19, CD33, CD34, CD38, CD41, CD45, CD56, CD61, CD64, CD71
, CD117, CD123, and HLA-DR was used for MRD detection, and 0.2-1 million events per tube were acquired on a Fluorescence activated cell sorter (FACS Canto II) (BD Co., USA).
The gene of Oct-4, Rex-1, CD29, CD44, CD71
, and CD73 were identified by RT-PCR and immunofluorescence technique.
In addition, phenotypic characterisation of AMs and IMs in mice revealed differences in the expression levels of MHC classII, CD11b, CD14, CD45, CD54, CD68, CD71
, CD204, CD206, and Siglec-F [5-9].
The stem cell surface markers HLA-DR, CD34, CD45, CD31, CD14, CD19, CD71
, CD105, CD73, and CD90 were quantified using appropriate antibodies (Becton Dickinson and Co., NJ, USA) on a fluorescence-activated cell sorting system Gallios (Beckman Coulter, USA).
Surface functionalization of modified PLA NPs was also performed with two bioactive ligands CD71
mAb and EGFR polyclonal antibody.
When comparing these two subpopulations with regard to immunophenotype and in vitro behaviour, they found that both [Lin.sup.-][CD34.sup.+] and [Lin.sup.-] [CD340.sup.-] SP subpopulation had a similar phenotype with cells negative for CD38, Thy-1, CD33, CD45RA, and CD71
and intermediate expression levels of HLA-DR .
(c) Immunohistochemical (IHC) staining for the erythroid marker CD71
Previous studies (7) using whole blood from patients with cirrhosis on the waiting list for liver transplantation found an increased expression of intralymphocyte interleukin 2 (IL-2) and tumor necrosis factor alpha (TNF[alpha])  and a decreased expression of the transferrin receptor (CD71
) and CD25 (the interleukin 2 receptor subunit [alpha]) as compared to healthy volunteers.
The FC studies we conducted showed that CD38, CD71
, and CD30 were positive in 100% of PELs.
United States-based CytomX Therapeutics has advanced CX-2029, a probody drug conjugate aimed at CD71
, which is highly attractive for delivery of cytotoxic payloads to cancer cells, it was reported on Friday.