Striking was a vascular bundle of the intima subintima border zone, which showed a positive staining with laminin- antibody and CD68
On immunohistochemistry, RDD was positive for both S-100 and CD68
proteins but negative for CD1a.
CD163 and CD68
immunostains demonstrated intensely positive staining in the hyperplastic population of stromal cells of both secondary and tertiary villi, confirming their identity as stromal macrophages, or Hofbauer cells.
The histiocytes stain positively for the CD68
However, a dIF assay using NN-Ab01 and an antibody against CD68
(#M0814; Dako), a macrophage marker, showed WU-VP1 and CD68
single-positive cells but no double-positive cells.
The giant cells and pleomorphic polygonal cells were dyed as vimentin positive in immunohistochemical dye CD68
CD31 (Figure 1-F), CD68
, and factor VIII-related antigen (Figure 1-E) highlighted the lesion.
These histiocytes were positive for S-100 protein (figure 1), CD68
, HAM-56, and lysozyme, and they showed emperipolesis.
The bone marrow core biopsy showed normal cellular components and abundant hemophagocytic macrophage infiltration based on CD68
2C), CD99, smooth muscle antibody, and bcl-2, but not cytokeratin (Fig-2D), co-factor, epithelial membrane antigen, desmin, myogenic differentiation antigen 1, S-100, actin, or CD68
We used the following cDNAs: human LOX-1 (GenBank NM002543), SR-A (GenBank NM002445), CD36 (GenBankNM000072), SR-BI (GenBank NM005505), CD68
(GenBank NM001251), and dectin-1 (GenBank NM197947), which were subcloned into pcDNA6.